Human cytokine panel 2

Manufactured by BioLegend

The Human Cytokine Panel 2 is a multiplex immunoassay that allows for the simultaneous detection and quantification of multiple human cytokines and chemokines from a single sample. The panel includes a selection of analytes relevant to immune system function and response.

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Lab products found in correlation

Mouse immunoglobulin isotyping panel, by BioLegend (2 mentions) Legendplex mouse th cytokine panel, by BioLegend (2 mentions) Human th cytokine panel, by BioLegend (2 mentions) Prism 7, by GraphPad (2 mentions) Human proinflammatory chemokine panel, by BioLegend (1 mentions) Legendplex, by BioLegend (1 mentions) Legendplex pro inflammatory chemokine panel, by BioLegend (1 mentions)

5 protocols using human cytokine panel 2

Cytokine Profiling in Patient Serum

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Serum samples of patients on day 1, day 3, day 5, day 6, and day 7 were investigated. Levels of different cytokines including IL-1β, IL-18, IL-6, tumor necrosis factor-α (TNF-α), monocyte chemoattractant protein-1 (MCP-1), interferon-γ (IFN-γ), IL-12p70, IL-8, IL-10, thymic stromal lymphopoietin (TSLP), IL-1α, granulocyte macrophage colony stimulating factor (GM-CSF), IFN-α2, IL-23, IL-12p40, IL-15, IL-11, IL-27, IL-33, and IL-17A were determined using the legendplex multi-analyte flow assay kit human inflammation panel and human cytokine panel 2 (BioLegend). Assays were performed according to the manufacturer’s instructions.

Cui J., Oehrl S., Ahmad F., Brenner T., Uhle F., Nusshag C., Rupp C., Funck F., Meisel S., Weigand M.A., Morath C, & Schäkel K. (2021). Detection of In Vivo Inflammasome Activation for Predicting Sepsis Mortality. Frontiers in Immunology, 11, 613745.

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Cytokine and Immunoglobulin Profiling

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Cytokines and immunoglobulin in human and mouse plasma were measured with the LEGENDplexTM mouse Th cytokine panel (740005, Biolegend), mouse immunoglobulin isotyping panel (740493, Biolegend), human cytokine panel 2 (740102, Biolegend), human Th cytokine panel (740721, Biolegend), and human proinflammatory chemokine panel (740003, Biolegend) following the manufacturers’ instructions. Data analysis was carried out using LEGENDplex™ Data Analysis Software (version 8.0). Heatmaps were constructed using the Graphpad Prism 7, and Venn diagrams completed on http://bioinformatics.psb.ugent.be/.

Li P., Jiang M., Li K., Li H., Zhou Y., Xiao X., Xu Y., Krishfield S., Lipsky P.E., Tsokos G.C, & Zhang X. (2021). Glutathione peroxidase 4 regulated neutrophil ferroptosis induces systemic autoimmunity. Nature immunology, 22(9), 1107-1117.

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Cytokine and Immunoglobulin Profiling

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Multiplex Cytokine Quantification

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Cytokines were quantified according to the manufacturer's instructions using the following BioLegend LEGENDplex™ kits: Human pro-inflammatory Chemokine panel (Cat No. 740003, lot B 254807), Human Cytokine Panel 2 (Cat. No 74010 2, Lot B274719), and Human Th Cytokine Panel (Cat. No 740722, Lot B260858). The absolute concentration of each factor was determined using the LEGENDplex™ data analysis software based on a standard curve recorded for each factor and run.
The LEGENDplex™ assay captures molecules through beads coated with specific antibodies. Specific biotinylated antibodies then bind to the captured molecules and are recognized by dye-coupled streptavidin. The dye signal intensities are quantified in a flow cytometer.

Wagner N., Eberhardt M., Vera J., Cuomo F., Blume K., Galster S., Achenbach S., Laffert B., Kahlert H., Schuler G., Berking C, & Baur A. (2021). Plasma-derived extracellular vesicles discriminate type-1 allergy subjects from non-allergic controls. The World Allergy Organization Journal, 14(9), 100583.

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Cytokine Profiling of Co-Cultures

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The supernatants of different co-cultures were screened for cytokine levels using the Legendplex pro-inflammatory Chemokine panel, human Cytokine panel 2, and anti-virus response panel (Biolegend).

Funck F., Pahl J., Kyjacova L., Freund L., Oehrl S., Gräbe G., Pezer S., Hassel J.C., Sleeman J., Cerwenka A, & Schäkel K. (2020). Human innate immune cell crosstalk induces melanoma cell senescence. Oncoimmunology, 9(1), 1808424.

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