Example 1
The naturally attenuated asymptomatic human bovine reassortant rotavirus strain 116E (serotype G9P[11]) used in this development had ingredients like sodium chloride, monosodium glutamate, Tris base, sucrose, and sodium bicarbonate reagents obtained from Fisher Bioreagents. DMEM and MEM growth media was purchased from Corning Life Sciences (Tewksbury, Mass.). Trizma, Hepes buffer and L-Arginine were obtained from Sigma Aldrich Saint Louis, Mo. Sodium citrate anhydrous powder (ADM, Chicago, Ill.) and Sodium adipate was from TCI America (Portland, Oreg.). Although the examples refer to immunogenic compositions of attenuated Rotavirus, the formulations are applicable to other types of virus including, but not limited to the non-enveloped viruses such as norovirus, adenovirus, papilloma virus, and picornavirus, and the enveloped viruses, Hepatitis virus, Corona virus, Influenza virus, and rabies virus.