Anti-IL-1β rabbit polyclonal antibody (cat. no. AF5103), glial fibrillary acidic protein (GFAP) rabbit polyclonal antibody (cat. no. DF6040), p-GSK3β (S9) rabbit polyclonal antibody (cat. no. AF2016), GSK3β rabbit polyclonal antibody (cat. no. AF5016), Caspase 1 rabbit polyclonal antibody (cat. no. AF5418), cleaved-Caspase 1 rabbit polyclonal antibody (cat. no. AF4005), and β-actin rabbit antibody (cat. no. AF7018) were obtained from Affinity Biosciences, Ltd. Nrf2 rabbit polyclonal antibody (cat. no. A1244), NLRP3 rabbit polyclonal antibody (cat. no. A5652), and NF-κB rabbit polyclonal antibody (cat. no. A19653) were purchased from ABclonal Biotech Co., Ltd. Hematoxylin and eosin (H&E) staining solution (cat. no. BL735B) was purchased from Biosharp Life Sciences. Lycorine was purchased from Shanghai yuanye Bio-Technology. The secondary antibody used for western blotting was an HRP Goat anti-rabbit IgG (H+L) (cat. no. AS014, ABclonal Biotech Co., Ltd.). The secondary antibody used for immunofluorescence analysis was a Goat Anti-Rabbit IgG H&L (FITC) (cat. no. ab6717, Abcam).
Lycorine
Manufactured by Yuanye Bio-Technology
Sourced in China
Lycorine is a laboratory compound used for research and analytical purposes. It is a crystalline substance derived from plant sources. Lycorine serves as a tool for scientific investigation and analysis, with specific applications determined by the intended research objectives.
Automatically generated - may contain errors
Lab products found in correlation
P akt, by Cell Signaling Technology (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Active caspase 3, by Cell Signaling Technology (1 mentions)
Enhanced chemiluminescence ecl detection system, by Merck Group (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
4e bp1, by Cell Signaling Technology (1 mentions)
P mtor, by Cell Signaling Technology (1 mentions)
3 protocols using lycorine
1
Antibody-Based Neuroinflammation Assessment
2
Cell Line Characterization and STAT3 Modulation
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Human CRC cell lines SW480 and RKO cell were obtained from ATCC in April 2017. The used cells were resuscitated within 1 month. The cell lines were identified by PCR-amplified short tandem repeat analysis. Mycoplasma contamination was excluded in these cell lines. Cells were maintained at 37°C in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin in a humidified atmosphere with 5% CO2. Expression vector of human STAT3 was designed and purchased from Servicebio Technologies (Wuhan, China). For siRNA knockdown, siRNA oligos against Bax was purchased respectively from GenePharma (Shanghai, China). Lycorine (purity > 98%) was purchased from Shanghai Yuanye Bio-Technology (Shanghai, China). A 20 mM stock solution was prepared in dimethyl sulfoxide (DMSO), and stored in aliquots at −20°C. Z-VAD-FMK was obtained from Calbiochem (San Diego, CA, United States). MTT [3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide] reagent was purchased from Sigma-Aldrich (St. Louis, MO, United States).
3
Lycorine-Induced Apoptosis Signaling
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Lycorine (purity > 98%) was purchased from Shanghai Yuanye Bio-Technology (Shanghai, China). A 40 mM stock solution was prepared in Dimethyl sulfoxide (DMSO) and stored at -20°C in aliquots. Dimethyl sulfoxide (DMSO) was purchased from Sigma (St. Louis, MO, USA). RPMI-1640 medium, fetal calf serum (FCS), L-glutamine were purchased from GIBCO Invitrogen Life Technologies (Carlsbad, USA). Primary antibodies against AKT, p-AKT, mTOR, p-mTOR, 4EBP1, Bax, Bcl-2, Active caspase-3 and actin were purchased from Cell Signaling Technology, Inc. (Beverly, USA). The enhanced chemiluminescence (ECL) detection system was obtained from Millipore. (Millipore, USA).
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