Microinjection system

After the experimental mice were anesthetized by isoflurane inhalation, they were placed on a digital stereotaxic instrument (Stoelting Co., United States), the heads of the mice were fixed, and the balance was adjusted using anterior tooth adapters and ear bars on both sides. Their skin was cut, and their skulls were exposed. The mouse hippocampal CA1 area coordinates were AP-1.9 mm, ML ± 1.4 mm, DV-1.3 mm, and the hole punch was lightly drilled. After redetermining the position, a microinjection system (Stoelting Co., United States) was used to control a glass electrode microinjector to slowly inject rAAV-EF1a-IFITM3-P2A-mCherry or rAAV-EF1a-mCherry into the bilateral hippocampal CA1 area. The total volume was 300 nL, and the speed was 23 nL/min for about 15 min. After the microinjection stopped, there was a 10 min wait to allow the virus to spread evenly to the tissue. After the needle was slowly pulled out, the head skin was sutured, the surgical area was smeared with aneriodine after surgery, 0.1 mL of 4% ropivacaine was injected locally into the incision, and lidocaine ointment was applied. After the mice were awake for 1 h, they were put back into their cage and were free to move around and eat and drink water.

After the experimental mice were anesthetized by isoflurane inhalation, they were placed on a digital stereotaxic instrument (Stoelting Co., United States), the heads of the mice were fixed, and the balance was adjusted using anterior tooth adapters and ear bars on both sides. Their skin was cut, and their skulls were exposed. The mouse hippocampal CA1 area coordinates were AP-1.9 mm, ML ± 1.4 mm, DV-1.3 mm, and the hole punch was lightly drilled. After redetermining the position, a microinjection system (Stoelting Co., United States) was used to control a glass electrode microinjector to slowly inject rAAV-EF1a-IFITM3-P2A-mCherry or rAAV-EF1a-mCherry into the bilateral hippocampal CA1 area. The total volume was 300 nL, and the speed was 23 nL/min for about 15 min. After the microinjection stopped, there was a 10 min wait to allow the virus to spread evenly to the tissue. After the needle was slowly pulled out, the head skin was sutured, the surgical area was smeared with aneriodine after surgery, 0.1 mL of 4% ropivacaine was injected locally into the incision, and lidocaine ointment was applied. After the mice were awake for 1 h, they were put back into their cage and were free to move around and eat and drink water.