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Anti syt7

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Anti-syt7 is a laboratory reagent that specifically binds to and detects the synaptotagmin-7 (syt7) protein. Synaptotagmin-7 is a calcium-binding protein involved in the regulation of synaptic vesicle fusion and neurotransmitter release. Anti-syt7 can be used in various biochemical and cell-based assays to study the localization, expression, and function of syt7 in biological samples.

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Lab products found in correlation

Fluoview 1200, by Olympus (2 mentions) Anti calbindin d 28k, by Merck Group (2 mentions) Anti vglut1, by Synaptic Systems (2 mentions) Halt protease inhibitor mixture, by Thermo Fisher Scientific (1 mentions) Anti syt1, by Synaptic Systems (1 mentions) Chemidoc reader, by Bio-Rad (1 mentions) Anti gapdh, by Merck Group (1 mentions)

3 protocols using anti syt7

1

Immunoblotting of Synaptic Proteins

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Cortical neurons at 16 DIV were harvested in RIPA lysis buffer [50 mM Tris⋅HCl, pH 7.5, 150 mM NaCl, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 1 mM EDTA, 1 mM EGTA] containing Halt protease inhibitor mixture (1 in 100; Thermo Fisher). After centrifugation at 16,000 × g for 15 min at 4 °C, samples were loaded onto an SDS/polyacrylamide gel, transferred to nitrocellulose membranes, and immunoblotted with primary antibodies followed by HRP-conjugated secondary antibodies. The following primary antibodies were used: anti-Syt1 (105 003; Synaptic Systems), anti-Syt7 (105 173; Synaptic Systems), and anti-GAPDH (glyceraldehyde 3-phosphate dehydrogenase; G8795; Sigma). Immunoreactive bands were visualized by enhanced chemiluminescence (ECL), captured using a Bio-Rad ChemiDoc reader, and analyzed using ImageLab software. Syt1 and Syt7 protein levels were normalized to the GAPDH loading control.
Tagliatti E., Bello O.D., Mendonça P.R., Kotzadimitriou D., Nicholson E., Coleman J., Timofeeva Y., Rothman J.E., Krishnakumar S.S, & Volynski K.E. (2020). Synaptotagmin 1 oligomers clamp and regulate different modes of neurotransmitter release. Proceedings of the National Academy of Sciences of the United States of America, 117(7), 3819-3827.
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2

Immunohistochemistry of Synaptic Proteins

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Two to four weeks after AAV injection, mice were anesthetized with ketamine and transcardially perfused with 4% PFA in PBS. The brain was removed and post-fixed for 24 hours. Slices (50 μm thick) were permeabilized (PBS + 0.4% Triton X-100) for 30 minutes and then prepared in blocking solution (PBS + 0.2% Triton X-100 + 2% normal goat serum [PBST]) for 30 min at room temperature. Slices were incubated overnight at 4°C in PBST with primary antibodies (anti-syt7 [Synaptic Systems, 105173], 1 μg/ml; 1:200, targeting AA 46–133 of syt7α, anti-vGlut1 [Synaptic Systems, 135304], 1 μg/ml; 1:500, and anti-calbindin-D28k [Sigma Aldrich, C9848], 1 μg/ml; 1:500), followed by incubation with secondary antibodies in PBST for 2 hr at room temperature. For both WT and syt7 KO mice, images from each brain region were acquired on a laser scanning confocal (Olympus, FluoView1200) using the same laser/microscope settings and processed in ImageJ identically.
Jackman S.L., Turecek J., Belinsky J.E, & Regehr W.G. (2016). The calcium sensor synaptotagmin 7 is required for synaptic facilitation. Nature, 529(7584), 88-91.
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3

Immunohistochemistry of Synaptic Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two to four weeks after AAV injection, mice were anesthetized with ketamine and transcardially perfused with 4% PFA in PBS. The brain was removed and post-fixed for 24 hours. Slices (50 μm thick) were permeabilized (PBS + 0.4% Triton X-100) for 30 minutes and then prepared in blocking solution (PBS + 0.2% Triton X-100 + 2% normal goat serum [PBST]) for 30 min at room temperature. Slices were incubated overnight at 4°C in PBST with primary antibodies (anti-syt7 [Synaptic Systems, 105173], 1 μg/ml; 1:200, targeting AA 46–133 of syt7α, anti-vGlut1 [Synaptic Systems, 135304], 1 μg/ml; 1:500, and anti-calbindin-D28k [Sigma Aldrich, C9848], 1 μg/ml; 1:500), followed by incubation with secondary antibodies in PBST for 2 hr at room temperature. For both WT and syt7 KO mice, images from each brain region were acquired on a laser scanning confocal (Olympus, FluoView1200) using the same laser/microscope settings and processed in ImageJ identically.
Jackman S.L., Turecek J., Belinsky J.E, & Regehr W.G. (2016). The calcium sensor synaptotagmin 7 is required for synaptic facilitation. Nature, 529(7584), 88-91.
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