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2 protocols using folin ciocalteu reagent lr

1

Quantification of Phytochemicals in Extracts

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Gallic acid monohydrate (≥98.0%), caffeine (anhydrous, 99%), (−)-epigallocatechin-3-gallate (≥95%), (−)-epicatechin-3-gallate (≥95%, HPLC), (−)-epigallocatechin (≥95%, HPLC), (−)-epicatechin (≥90%, HPLC), (+)-catechin (≥95%, HPLC) and l-theanine (≥98%, HPLC) were procured from Sigma Aldrich, India. Folin–Ciocalteu reagent (LR) was purchased from Himedia (HiMedia Laboratories, India). Acetic acid (HPLC Grade), acetonitrile (HPLC Grade), sodium carbonate and all other chemicals were obtained from Merck KGaA, Darmstadt, Germany.
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2

Phytochemical Standards Characterization

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Gallic acid monohydrate (≥98.0%), (−)-epigallocatechin-3-gallate (≥95%),(−)-epigallocatechin (≥95%, HPLC), (−)-epicatechin-3-gallate (≥95%, HPLC), (+)-catechin (≥95%, HPLC), (−)-epicatechin (≥90%, HPLC), caffeine (anhydrous, 99%), theaflavin (≥90%, HPLC), theaflavin-3-gallate (≥95%, HPLC), theaflavin-3′-gallate (≥90%, HPLC), theaflavin-3,3′-digallate (≥90%, HPLC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), potassium persulfate, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) and iron(iii) chloride hexa-hydrate were purchased from Sigma-Aldrich, India. Folin-Ciocalteu reagent (LR) was obtained from Himedia (HiMedia Laboratories, India). Acetonitrile (HPLC grade), acetic acid (HPLC grade), sodium carbonate and all other chemicals were procured from Merck KGaA, Darmstadt, Germany.
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