Adult C57Bl6J mice were perfused transcardially with ice-cold PBS followed by 10% formalin. Brains were post-fixed 24 hours in 10% formalin, cryopreserved in 30% sucrose, and then sliced into 35 μm sections. Immunohistochemistry was performed using a rabbit anti-HA antibody to detect virally overexpressed SGK1 (Cell Signaling C29F4, Lot 9) and a goat anti-GFP antibody (Abcam Ab5450) and secondary antibodies: Alexa-Flour 488 anti-goat IgG (Jackson; 705-545-147 1:200) and Cy3 anti-rabbit IgG (Jackson; 711-165-152 1:200).
Alexa flour 488 anti goat igg
Manufactured by Jackson ImmunoResearch
Alexa-Fluor 488 anti-goat IgG is a fluorescently labeled secondary antibody that specifically binds to goat immunoglobulin G (IgG). Alexa-Fluor 488 is a bright, green-fluorescent dye that can be used for immunofluorescence microscopy, flow cytometry, and other fluorescence-based applications.
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Lab products found in correlation
2 protocols using alexa flour 488 anti goat igg
1
Immunohistochemistry of Virally Expressed SGK1
2
Immunostaining of GFP and Androgen Receptor
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