Rsc simplyrna tissue

Manufactured by Promega

Sourced in United States

The RSC simplyRNA Tissue is a laboratory equipment product that is designed for the extraction of high-quality RNA from tissue samples. It provides a streamlined and efficient process for RNA isolation, allowing researchers to obtain reliable and reproducible results.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (4 mentions) Cfx384 touch real time pcr detection system, by Bio-Rad (2 mentions) M mlv rt kit, by Promega (1 mentions) Maxwell rsc instrument, by Promega (1 mentions) M mlv reverse transcriptase, by Promega (1 mentions)

Close spelling variants of this product

Maxwell RSC simplyRNA Tissue Kit (107 citations) OrMaxwell® RSC simplyRNA Tissue Kit (2 citations)

2 protocols using rsc simplyrna tissue

Gene Expression Analysis by RT-qPCR

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Total RNA extraction was performed by using TRIzol (#15596026, Thermo Fischer Scientific) or by using a Maxwell RSC instrument (#AS1340, RSC simplyRNA Tissue, Promega, Madison, WI, USA). Reverse transcription of total RNA (1 µg) was carried out using the MMLV-RT kit (#M3683, Promega) with random hexamers. Real-time quantitative PCR (RT-qPCR) was subsequently performed with a 1:10 dilution of reverse-transcribed cDNA using a CFX384 Touch™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA). RT-qPCR TagMan Universal Master Mix II (#4440038, Thermo Fischer Scientific) was employed, with TagMan probes specific for HOTAIRM1- (exon 1-3, #Hs.PT.58.45434173, Integrated DNA Technologies (IDT), Coralville, IA, USA), TGM2 (#Hs.PT.58.23141755, IDT) or PGK1 (#Hs.PT.58 v.606641, IDT). RT-qPCR results were evaluated by 2^−ΔΔCt method [27 (link)] using PGK1 expression levels as a housekeeping gene control.

Ahmadov U., Picard D., Bartl J., Silginer M., Trajkovic-Arsic M., Qin N., Blümel L., Wolter M., Lim J.K., Pauck D., Winkelkotte A.M., Melcher M., Langini M., Marquardt V., Sander F., Stefanski A., Steltgens S., Hassiepen C., Kaufhold A., Meyer F.D., Seibt A., Kleinesudeik L., Hain A., Münk C., Knobbe-Thomsen C.B., Schramm A., Fischer U., Leprivier G., Stühler K., Fulda S., Siveke J.T., Distelmaier F., Borkhardt A., Weller M., Roth P., Reifenberger G, & Remke M. (2021). The long non-coding RNA HOTAIRM1 promotes tumor aggressiveness and radiotherapy resistance in glioblastoma. Cell Death & Disease, 12(10), 885.

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RNA Extraction and Real-Time PCR Quantification

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Extraction of RNA from treated or untreated cells was conducted using Trizol (#15 596–018, Thermo Scientific) or the Maxwell RSC Instrument (RSC simplyRNA Tissue, #AS1340, Promega), and complementary DNA (cDNA) was synthesized from 0.5 µg RNA using M-MLV Reverse Transcriptase (#M3683, Promega) according to the manufacturer’s instructions. Quantitative real-time PCR was performed using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad, Munich, Germany) with TaqMan probes for MYC (Hs.PT.58.26770695, IDT, Leuven, Belgium) and TGM2 (Hs.PT.58.23141755, IDT). Samples were amplified in triplicate, and relative quantification to housekeeping genes PPIA (Hs.PT.39a.22214851, IDT) and PGK1 (Hs.PT.58.606641, IDT) was assessed using the ΔΔC_T method.

Marquardt V., Theruvath J., Pauck D., Picard D., Qin N., Blümel L., Maue M., Bartl J., Ahmadov U., Langini M., Meyer F.D., Cole A., Cruz-Cruz J., Graef C.M., Wölfl M., Milde T., Witt O., Erdreich-Epstein A., Leprivier G., Kahlert U., Stefanski A., Stühler K., Keir S.T., Bigner D.D., Hauer J., Beez T., Knobbe-Thomsen C.B., Fischer U., Felsberg J., Hansen F.K., Vibhakar R., Venkatraman S., Cheshier S.H., Reifenberger G., Borkhardt A., Kurz T., Remke M, & Mitra S. (2023). Tacedinaline (CI-994), a class I HDAC inhibitor, targets intrinsic tumor growth and leptomeningeal dissemination in MYC-driven medulloblastoma while making them susceptible to anti-CD47-induced macrophage phagocytosis via NF-kB-TGM2 driven tumor inflammation. Journal for Immunotherapy of Cancer, 11(1), e005871.

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