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Islet capture microplates

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Islet Capture Microplates are a specialized laboratory equipment designed for the isolation and purification of pancreatic islets. They provide a controlled environment for the capture and retrieval of islets, which are essential for research and clinical applications related to diabetes and islet transplantation.

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Lab products found in correlation

D5030, by Merck Group (2 mentions) Biopsy puncher, by Merck Group (2 mentions)

2 protocols using islet capture microplates

1

Adipose Tissue Metabolic Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Subcutaneous white adipose tissue (1.5-2 mg) was obtained using a Biopsy Puncher (2 mm) and placed into XF24 Islet Capture Microplates and incubated in assay medium (substrate-free DMEM (Sigma D5030), 30 mM NaCl, pH7.4, 25 mM glucose, 0.5 mM sodium pyruvate). Oxygen consumption rate was measured on a Sehaorse XF24 Flux analyser following sequential addition of oligomycin (5 μM), carbonyl cyanide 3-chlorophenyl hydrazone (CCCP, 5 μM) and antimycin (5 μM).
Pollard A.E., Martins L., Muckett P.J., Khadayate S., Bornot A., Clausen M., Admyre T., Bjursell M., Fiadeiro R., Wilson L., Whilding C., Kotiadis V.N., Duchen M.R., Sutton D., Penfold L., Sardini A., Bohlooly-Y M., Smith D.M., Read J.A., Snowden M.A., Woods A, & Carling D. (2019). AMPK activation protects against diet induced obesity through Ucp1-independent thermogenesis in subcutaneous white adipose tissue. Nature metabolism, 1(3), 340-349.
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2

Adipose Tissue Metabolic Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Subcutaneous white adipose tissue (1.5-2 mg) was obtained using a Biopsy Puncher (2 mm) and placed into XF24 Islet Capture Microplates and incubated in assay medium (substrate-free DMEM (Sigma D5030), 30 mM NaCl, pH7.4, 25 mM glucose, 0.5 mM sodium pyruvate). Oxygen consumption rate was measured on a Sehaorse XF24 Flux analyser following sequential addition of oligomycin (5 μM), carbonyl cyanide 3-chlorophenyl hydrazone (CCCP, 5 μM) and antimycin (5 μM).
Pollard A.E., Martins L., Muckett P.J., Khadayate S., Bornot A., Clausen M., Admyre T., Bjursell M., Fiadeiro R., Wilson L., Whilding C., Kotiadis V.N., Duchen M.R., Sutton D., Penfold L., Sardini A., Bohlooly-Y M., Smith D.M., Read J.A., Snowden M.A., Woods A, & Carling D. (2019). AMPK activation protects against diet induced obesity through Ucp1-independent thermogenesis in subcutaneous white adipose tissue. Nature metabolism, 1(3), 340-349.
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