Zetasizer nano range

Manufactured by Malvern Panalytical

Sourced in United Kingdom

The Zetasizer Nano Range is a series of dynamic light scattering (DLS) instruments designed for the measurement of particle size, zeta potential, and molecular weight. The instruments use non-invasive back-scatter technology to characterize samples in a wide range of concentrations and sizes, from sub-nanometer to micron-scale particles and molecules.

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Lab products found in correlation

S 4800 scanning electron microscope, by Hitachi (1 mentions) Gp100, by Abcam (1 mentions) Trp 2, by Santa Cruz Biotechnology (1 mentions) Micro bca assay kit, by Thermo Fisher Scientific (1 mentions)

5 protocols using zetasizer nano range

Dynamic Light Scattering Analysis of Pillar-1c

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Samples of the Pillar-1c at different concentrations (2 and 50 mM in CHCl₃) were analyzed on a Zetasizer Nano Range, Malvern PANalytical (UK) at 298 K. All DLS measurements were performed at a scattering angle of 90°. Sample solutions were prepared by filtering each component solution through a 0.2 μm polytetrafluoroethylene (PTFE) syringe filter into a clean scintillation vial.

Al-Azemi T.F, & Vinodh M. (2021). Concentration-dependent supramolecular self-assembly of A1/A2-asymmetric-difunctionalized pillar[5]arene. RSC Advances, 11(5), 2995-3002.

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Nanoparticle Characterization by DLS and SEM

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Nano-PAC was diluted 1,000 times in deionized water and further filtered with 0.22 µm membranes. Each 700 µL of sample was placed in a quartz tube and measured for the averaged particle diameter and zeta potential by Zetasizer nano range (Malvern Instruments, Malvern, UK). Hitachi S-4800 scanning electron microscope (SEM; Hitachi Ltd., Tokyo, Japan) was used to observe the particle size and morphology of NPs, and the micrographs were taken at 15 kV.31

Sudirman S., Hsu Y.H., Johnson A., Tsou D, & Kong Z.L. (2018). Amelioration effects of nanoencapsulated triterpenoids from petri dish-cultured Antrodia cinnamomea on reproductive function of diabetic male rats. International Journal of Nanomedicine, 13, 5059-5073.

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Nanoparticle stability assessment

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Zeta potential and hydrodynamic diameter measurements were conducted using the Malvern Zetasizer Nano Range. The as-synthesized GBPs were diluted to 5 percent of a starting concentration that provided a UV–vis absorbance value of OD 1 at the longitudinal LSPR peak. The pH of each sample was adjusted by addition of HCl or NaOH. pH values from 5 to 9 were used to measure the stability of the particles. Zeta potential measurements of functionalized and bioconjugated nanoparticles were taken with all samples at pH 7.

Abdul-Moqueet M.M., Tovias L., Lopez P, & Mayer K.M. (2021). Synthesis and bioconjugation of alkanethiol-stabilized gold bipyramid nanoparticles. Nanotechnology, 32(22), 10.1088/1361-6528/abe823.

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Dynamic Light Scattering Analysis of 1,3-BUP

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Samples of 1,3-BUP at
different concentrations (2, 5, and 10 mM in DMSO) were analyzed on
a Zetasizer Nano Range, Malvern PANalytical (U.K.) at 25 °C.
All DLS measurements were performed at a scattering angle of 90°.
Sample solutions were prepared by filtering each component solution
through a 0.2 μm poly(tetrafluoroethylene) (PTFE) syringe filter
into a clean scintillation vial.

Vinodh M., Alipour F.H, & Al-Azemi T.F. (2022). Spatially Designed Supramolecular Anion Receptors Based on Pillar[5]arene Scaffolds: Synthesis and Halide Anion Binding Properties. ACS Omega, 8(1), 1466-1475.

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Characterization of Protein-Loaded Nanoparticles

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Sample concentrations for all assays were standardized by the total protein content as measured by MicroBCA Assay Kit (Thermo Scientific). SDS-PAGE was performed, followed by gel staining with Coomassie or transfer to a PVDF membrane for Western Blotting using antibodies against gp100 (Abcam), TRP2 (Santa Cruz), or ovalbumin (Abcam). Transmission electron microscopy images were obtained using JEOL 1400-plus microscope (JOEL USA) following sodium phosphotungstate negative staining. Particle size and zeta potential were measured and analyzed using dynamic light scattering (DLS, Malvern Zetasizer Nano Range) in PBS or ultrapure water, respectively. For stability studies, samples were incubated with PBS or 10% FBS in PBS at 4 °C (long term) or at 37 °C while shaking (short term) as indicated in the figures.

Ochyl L.J., Bazzill J.D., Park C., Xu Y., Kuai R, & Moon J.J. (2018). PEGylated tumor cell membrane vesicles as a new vaccine platform for cancer immunotherapy. Biomaterials, 182, 157-166.

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