El 01125047 spectrophotometer

Unless mentioned, all chemicals and proteins were used as received from Sigma-Aldrich. ¹H-NMR and ¹³C-NMR spectra were recorded on a 400 MHz Bruker NMR spectrometer. Molecular weight of the polymers was measured by gel permeation chromatography (GPC, Waters) using a PMMA standard with a refractive index detector and THF as eluent with a flow rate of 1 mL/min. Dynamic light scattering (DLS) measurements were performed using a Malvern Nanozetasizer. UV-visible absorption spectra were recorded on a Varian (model EL 01125047) spectrophotometer. The fluorescence spectra were obtained using a JASCO FP-6500 spectrofluorimeter. Fluorescent images were recorded on Nikon with Yokogawa spinning disk (SD) and Nikon A1 spectral detector confocal with FLIM module.

¹H NMR and ¹³C NMR spectra were recorded on a 400 MHz Bruker NMR spectrometer. Molecular weight of the polymers was measured by gel permeation chromatography (GPC, Waters) using a PMMA standard with a refractive index detector. THF was used as eluent with a flow rate of 1 mL/min. Dynamic light scattering (DLS) measurements were performed using a Malvern Nanozetasizer. TEM images were recorded in JEOL 2000FX transmission electron microscope. UV-visible absorption spectra were recorded on a Varian (model EL 01125047) spectrophotometer. The fluorescence spectra were obtained from a JASCO FP-6500 spectrofluorimeter.