DNA from ChIP was quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q33230). Sequencing libraries were created according to the ThruPLEX DNA-seq kit protocol (Takara, R400676). Size selection was performed in the range of 200 to 700 bp with AMPure XP beads (Beckman Coulter, A63880) and confirmed using the Agilent High Sensitivity DNA Kit (Agilent, 5067-4626) on the Agilent 2100 Bioanalyzer. Libraries were pooled and sequenced using the NextSeq 500/550 High Output Kit v2.5 (Illumina, 20024906). The sequencing run was single end and dual index with 75-bp reads. For the total RNAPII and TOP1 ChIP-Rx-seq, the libraries were pooled and sequenced using the NextSeq 1000/2000 P2 Reagents (100 cycles) v3 kit (Illumina, 20046811). These sequencing runs were single end and dual index with 115-bp reads.
Nextseq 1000 2000 p2 reagents 100 cycles v3 kit
Manufactured by Illumina
The NextSeq 1000/2000 P2 Reagents (100 cycles) v3 kit is a sequencing reagent kit designed for use with the NextSeq 1000 and NextSeq 2000 sequencing systems. The kit provides the necessary reagents for performing 100 cycles of sequencing.
Automatically generated - may contain errors
Lab products found in correlation
Agilent high sensitivity dna kit, by Agilent Technologies (2 mentions)
Nextseq 500 550 high output kit v2, by Illumina (2 mentions)
Qubit dsdna hs assay kit, by Thermo Fisher Scientific (2 mentions)
Ampure xp bead, by Beckman Coulter (2 mentions)
Thruplex dna seq kit protocol, by Takara Bio (2 mentions)
2 protocols using nextseq 1000 2000 p2 reagents 100 cycles v3 kit
1
ChIP-Seq Library Preparation and Sequencing
2
ChIP-Seq Library Preparation and Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DNA from ChIP was quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Q33230). Sequencing libraries were created according to the ThruPLEX DNA-seq kit protocol (Takara, R400676). Size selection was performed in the range of 200 to 700 bp with AMPure XP beads (Beckman Coulter, A63880) and confirmed using the Agilent High Sensitivity DNA Kit (Agilent, 5067-4626) on the Agilent 2100 Bioanalyzer. Libraries were pooled and sequenced using the NextSeq 500/550 High Output Kit v2.5 (Illumina, 20024906). The sequencing run was single end and dual index with 75-bp reads. For the total RNAPII and TOP1 ChIP-Rx-seq, the libraries were pooled and sequenced using the NextSeq 1000/2000 P2 Reagents (100 cycles) v3 kit (Illumina, 20046811). These sequencing runs were single end and dual index with 115-bp reads.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!