α beta actin

Manufactured by Merck Group

Sourced in United States

α-beta actin is a cytoskeletal protein that plays a crucial role in various cellular processes. It is a key component of the actin filaments, which are responsible for maintaining the structural integrity of cells and facilitating cellular movement and division. This protein is highly conserved across different species and is commonly used as a reference or control in various biological research applications.

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Lab products found in correlation

α lc3b, by Cell Signaling Technology (1 mentions) Donkey α goat, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg hrp, by Merck Group (1 mentions) Alexa fluor 488 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg hrp, by Merck Group (1 mentions) α dsrna, by Techcomp Instruments (1 mentions) Alexa fluor 568 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Prolong gold antifade reagent, by Cell Signaling Technology (1 mentions) Alexa fluor 647 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) α alpha tubulin, by Merck Group (1 mentions) Odyssey infrared imager, by LI COR (1 mentions) α pgc1α, by Abcam (1 mentions)

Spelling variants of this product

α-actin (101 citations)

4 protocols using α beta actin

Autophagy Marker Detection Antibodies

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Rabbit polyclonal antibodies were α-p62 (Cliniscience, PM045), α-NDP52 (AbCam, ab68588), α-LC3B (AbCam ab48394), α-GABARAP (Abgent, AP1821a). Rabbit monoclonal antibodies were α-LC3B (Cell Signalling, 3868P for immunoblotting), α-ubiquitin Lys-63 specific, Apu3 (Merck Millipore, 05–1308), α-ubiquitin Lys-48 specific, Apu2 (Merck Millipore, 05–1307), α-ubiquitin M1 linear-specific, 1E3 (Merck Millipore, 199), α-Atg16L1 D6D5 (Cell Signalling, 8089), α-Rab7 D95F2 (Cell Signalling, 9367). Mouse monoclonal antibodies were α-ubiquitin FK2 (Enzo Life Sciences, PW8810), α-p62 (abcam, 56416), α-LAMP-1 H4A3 (Abcam ab25630), α-beta actin (Sigma, A2228). Goat polyclonal antibodies were α-galectin 8 (R&D systems, AF1305). Secondary antibodies used were Alexa Fluor 488-, or Alexa Fluor 568-, Alexa Fluor 647-conjugated chicken/goat α-rabbit, chicken/goat α-mouse or donkey α-goat (Molecular Probes).

Clough B., Wright J.D., Pereira P.M., Hirst E.M., Johnston A.C., Henriques R, & Frickel E.M. (2016). K63-Linked Ubiquitination Targets Toxoplasma gondii for Endo-lysosomal Destruction in IFNγ-Stimulated Human Cells. PLoS Pathogens, 12(11), e1006027.

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Primary and Secondary Antibodies for Viral Protein Detection

Cited 1 time

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Commercially available primary antibodies used in this study are as follows: α-Beta Actin (Sigma Aldrich: A5441), α-RTN3 (Santacruz: sc374599), α-PDI (Thermofisher: P7496), α-DENV NS3 (Genetex: GTX629477), α-DENV NS4B (Genetex: GTX124250), α-dsRNA (Scicons: 10010500), α-ZIKV NS2B (Genetex: GTX133318), α-ZIKV NS1 (Genetex: GTX634158), α-ZIKV NS3 (Genetex: GTX133320), α-ZIKV NS4A (Genetex: GTX133704), α-ZIKV NS4B (Genetex: GTX133321), α-ZIKV NS5 (Genetex: GTX133327). Commercially available secondary antibodies and IF reagents used in this study are as follows: Goat anti–rabbit IgG-HRP (Sigma Aldrich: A6154), Goat anti–mouse IgG-HRP (Sigma Aldrich: A4416), Alexa Fluor 488 donkey anti-mouse IgG (Thermofisher: A-21202), Alexa Fluor 488 donkey anti-mouse IgG2a (Thermofisher: A-21131), Alexa Fluor 568 donkey anti-rabbit IgG (Thermofisher: A-10042), Alexa Fluor 568 donkey anti-mouse IgG1 (Thermofisher: A-21124), Alexa Fluor 647 donkey anti-rabbit IgG (Thermofisher: A −31573), ProLong® Gold Antifade Reagent (Cell Signaling Technology: #9071) and DAPI (Sigma Aldrich: D9542). The DENV NS1-specific antibody was produced as described earlier (Welsch et al., 2009 (link)). DENV NS3-, NS4B- and NS5-specific antibodies were used for western blot analyses as described elsewhere (Miller et al., 2006 (link)).

Cerikan B., Goellner S., Neufeldt C.J., Haselmann U., Mulder K., Chatel-Chaix L., Cortese M, & Bartenschlager R. (2020). A Non-Replicative Role of the 3′ Terminal Sequence of the Dengue Virus Genome in Membranous Replication Organelle Formation. Cell Reports, 32(1), 107859.

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HIV-1 Protein Immunoblotting Protocol

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Virus-containing supernatants (normalised for equal loading by measuring RT activity) or 15 μg of total CD4+ T cell protein lysate were separated by SDS-PAGE, transferred onto nitrocellulose and blocked in PBS with 0.05% Tween 20 (v/v) and 5% skimmed milk (w/v). Blots were probed with rabbit antisera raised against HIV-1 Gag p24 (cat# 0432 donated by Dr G. Reid and obtained from the CFAR), Vpr anti-serum (cat# 3951, NIH ARP), α−P-STAT5 (Tyr694) (D47E7, Cell Signaling Technology), α-STAT5 (D3N2B, Cell Signaling Technology), α-beta-Actin (A2066, Sigma-Aldrich), α-UNG (OTI2C12, OriGene Technologies), α−alpha-Tubulin (T6199, Sigma-Aldrich) and α-DCAF1 antibody (11612-1-AO, Proteintech), followed by goat anti-rabbit or goat anti-mouse IRdye 800CW or 680RD infrared secondary antibody (Abcam) and imaged using an Odyssey Infrared Imager (LI-COR Biosciences) and analysed with Image Studio Lite software.

Reuschl A.K., Mesner D., Shivkumar M., Whelan M.V., Pallett L.J., Guerra-Assunção J.A., Madansein R., Dullabh K.J., Sigal A., Thornhill J.P., Herrera C., Fidler S., Noursadeghi M., Maini M.K, & Jolly C. (2022). HIV-1 Vpr drives a tissue residency-like phenotype during selective infection of resting memory T cells. Cell Reports, 39(2), 110650.

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Antibody Characterization for Cellular Analysis

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The following antibodies from Cell Signaling (USA) were used: monoclonal α-pAMPK, α-tyrosine hydroxylase and polyclonal α-TFAM. Antibodies from other manufacturers include monoclonal α-AMPK and polyclonal α-PGC-1α (Abcam, UK), monoclonal α-PRK8 (Signet/Covance, USA), α-beta-actin (Sigma, USA), α-PARIS (Merck Millipore, USA), α-mouse horseradish peroxidase and α-rabbit horseradish peroxidase (GE Healthcare, UK).

Hang L., Thundyil J., Goh G.W, & Lim K.L. (2018). AMP Kinase Activation is Selectively Disrupted in the Ventral Midbrain of Mice Deficient in Parkin or PINK1 Expression. Neuromolecular Medicine, 21(1), 25-32.

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