SENP1+/lox mice were generated by inserting loxP sites surrounding the SENP1 gene exons 5 and 6, based on homologous recombination17 (link). SENP1lox/lox mice were obtained by intercrossing SENP1+/lox mice. SENP1lox/lox mice were mated with three different deleter lines carrying the Cre recombinase driven by the Adiponectin (obtained from Jackson Laboratory), PdgfRα (obtained from Dr. Matthew Rodeheffer at Yale University School of Medicine), or aP2/Fabp4 gene promoter (BI line from Dr. Barbara Kahn, Beth Israel Deaconess Medical School, Harvard Medical School). All mice had been subsequently backcrossed onto the C57BL/6 background for >6th generations. The deletion of SENP1 in adipocytes of SENP1lox/lox :Cre was verified by qRT-PCR using primers amplifying exons 5–6 17 (link) and specific Cre. SENP1+/+:Cre and SENP1lox/lox mice used as controls. Mice were cared for in accordance with National Institutes of Health guidelines, and all procedures were approved by the Yale University Animal Care and Use Committee.
Pdgfrα
Manufactured by Jackson ImmunoResearch
PdgfRα is a receptor tyrosine kinase that binds to platelet-derived growth factor (PDGF). It plays a role in cell growth, proliferation, and differentiation.
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Lab products found in correlation
2 protocols using pdgfrα
1
Conditional Deletion of SENP1 in Mice
2
Generation and Validation of SENP1 Knockout Mice
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SENP1+/lox mice were generated by inserting loxP sites surrounding the SENP1 gene exons 5 and 6, based on homologous recombination17 (link). SENP1lox/lox mice were obtained by intercrossing SENP1+/lox mice. SENP1lox/lox mice were mated with three different deleter lines carrying the Cre recombinase driven by the Adiponectin (obtained from Jackson Laboratory), PdgfRα (obtained from Dr Matthew Rodeheffer at Yale University School of Medicine) or aP2/Fabp4 gene promoter (BI line from Dr Barbara Kahn, Beth Israel Deaconess Medical School, Harvard Medical School). All mice had been subsequently backcrossed onto the C57BL/6 background for >6th generations. The deletion of SENP1 in adipocytes of SENP1lox/lox:Cre was verified by quantitativePCR with reverse transcription using primers amplifying exons 5–6 (ref. 17 (link)) and specific Cre. SENP1+/+:Cre and SENP1lox/lox mice used as controls. Mice were cared for in accordance with National Institutes of Health guidelines, and all procedures were approved by the Yale University Animal Care and Use Committee.
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