Plan apochromat 63x na 1.40 oil immersion objective

Manufactured by Zeiss

The Plan-Apochromat 63X/NA 1.40 oil immersion objective is a high-performance microscope lens manufactured by Zeiss. It provides a magnification of 63X and a numerical aperture of 1.40, which enables high-resolution imaging. The objective is designed for use with oil immersion technique to achieve optimal optical performance.

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Lab products found in correlation

365 nm pulsed nitrogen laser, by Spectra-Physics (3 mentions) Axiovert 200m microscope, by Zeiss (3 mentions) Co2 independent medium, by Thermo Fisher Scientific (1 mentions) Axiocam hrm camera, by Zeiss (1 mentions) Jetprime, by Polyplus Transfection (1 mentions) Axiovision software version 4, by Zeiss (1 mentions)

3 protocols using plan apochromat 63x na 1.40 oil immersion objective

Real-Time Recruitment of DNA Repair Factors

Cited 2 times

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GFP-NBS1, GFP-EXO1, GFP-Ku70, GFP-XLF, or GFP-XRCC4 were transfected into HCT116 DNA-PK_cs +/+, −/−, or KD/− cells with JetPrime^® (Polyplus) following the manufacturer's instructions. Twenty-four hours after the transfection laser micro-irradiation and real-time recruitment was performed with a Carl Zeiss Axiovert 200M microscope with a Plan-Apochromat 63X/NA 1.40 oil immersion objective (Carl Zeiss) as previously described (28 (link)). DSBs were generated with a 365-nm pulsed nitrogen laser (Spectra-Physics), which was directly coupled to the epifluorescence path of the microscope (28 (link)). Time-lapse images were taken via a Carl Zeiss AxioCam HRm camera. The cells were maintained in a CO₂-independent medium (Invitrogen) at 37°C during micro-irradiation and time-lapse imaging. Fluorescence intensities of the micro-irradiated area and control area were determined by Carl Zeiss Axiovision software, v4.5, and the intensity of irradiated was normalized to non-irradiated control area as previously described (26 (link)).

Lu H., Saha J., Beckmann P.J., Hendrickson E.A, & Davis A.J. (2019). DNA-PKcs promotes chromatin decondensation to facilitate initiation of the DNA damage response. Nucleic Acids Research, 47(18), 9467-9479.

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Laser Micro-Irradiation for Live-Cell Imaging

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Live cell imaging combined with laser micro-irradiation was performed as described previously. Fluorescence signal of YFP-DNA-PKcs was monitored by using an Axiovert 200M microscope (Carl Zeiss, Inc), with a Plan-Apochromat 63X/NA 1.40 oil immersion objective (Carl Zeiss, Inc) [20 (link)]. A 365-nm pulsed nitrogen laser (Spectra Physics) was directly coupled to the epifluorescence path of the microscope and used to generate DSBs in a defined area of the nucleus. Analysis of acquired images was done as previously described [34 (link)]. Briefly, fluorescence intensity (IN) of each time point was based on pre-laser background intensity using the formula: IN(t) = Id_t/Ib_t×Ib_preIR [Id_t: the difference between the accumulation spot intensity and the undamaged site background intensity of each time point; Ib_t: the background intensity of each time point; Ib_preIR: the background intensity before irradiation. Relative fluorescence intensity (RF) was calculated using the formula: RF(t) = (IN_t−IN_preIR)/(IN_max−IN_preIR) [IN_preIR: IN of the micro-irradiated area before laser damage; IN_max: the maximum IN in the micro-irradiated area of all time points]. Each data point is the average of 10 independent measurements.

Mori E., Davis A.J., Hasegawa M, & Chen D.J. (2016). Lysines 3241 and 3260 of DNA-PKcs are important for genomic stability and radioresistance. Biochemical and biophysical research communications, 477(2), 235-240.

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Live-Cell Imaging and Laser Micro-Irradiation

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Live cell imaging combined with laser micro-irradiation was carried out as previously described (11 (link),29 (link)). Fluorescence was monitored via an Axiovert 200 M microscope (Carl Zeiss, Inc.), with a Plan-Apochromat 63X/NA 1.40 oil immersion objective (Carl Zeiss, Inc.). A 365-nm pulsed nitrogen laser (Spectra-Physics) was directly coupled to the epifluorescence path of the microscope and used to generate DSBs in a defined area of the nucleus. For quantitative analyses, the same amount of DNA damage was generated under standardized micro-irradiation conditions (minimal laser output of 75% for 5 pulses) in each experiment. Time-lapse images were taken with an AxioCamHRm camera. The fluorescence intensities of micro-irradiated and non-irradiated areas within the cell nucleus were determined using the AxioVision Software, version 4.8 (Carl Zeiss, Inc.). Each data point is the average of 10 independent measurements.

Lee K.J., Saha J., Sun J., Fattah K.R., Wang S.C., Jakob B., Chi L., Wang S.Y., Taucher-Scholz G., Davis A.J, & Chen D.J. (2015). Phosphorylation of Ku dictates DNA double-strand break (DSB) repair pathway choice in S phase. Nucleic Acids Research, 44(4), 1732-1745.

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