HeLa cells were cultured in modified Eagle’s medium- + glutamax (Gibco, 41090-028) supplemented with 10% fetal bovine serum, 1% penicillin, and 1% streptomycin. Cells were seeded and grown on 35-mm optical polymer-bottom dishes containing an etched grid (35 mm Grid-500 dishes, Ibidi, Switzerland) and transfected with 5 μl Lipofectamine 2000 (Thermo) and 2 μg mitoSypHer (Poburko & Demaurex, 2012 (link)). Cells were imaged in a modified Ringer’s solution containing 20 mM N-2-hydroxyethylpiperazine-N′-2-ethane sulfonic acid, 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, 2 mM CaCl2, 10 mM glucose, pH set to 7.4 at 37°C.
Modified eagle s medium glutamax
Manufactured by Thermo Fisher Scientific
Modified Eagle's medium- + glutamax is a cell culture medium formulation designed to support the growth and maintenance of a variety of mammalian cell lines. It is a modification of the original Eagle's Minimum Essential Medium, with the addition of the amino acid glutamine.
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Lab products found in correlation
2 protocols using modified eagle s medium glutamax
1
Visualizing Mitochondrial pH Dynamics in HeLa Cells
2
Visualizing Mitochondrial pH Dynamics in HeLa Cells
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