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Goat anti rabbit or anti mouse immunoglobulin g igg horseradish peroxidase hrp secondary antibodies

Manufactured by Bioss Antibodies
Sourced in China

Goat anti-rabbit or anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP) secondary antibodies are lab equipment used to detect the presence of primary antibodies that bind to rabbit or mouse antigens. These secondary antibodies are conjugated with the enzyme horseradish peroxidase, which can catalyze a colorimetric or chemiluminescent reaction for visualization and quantification purposes.

Automatically generated - may contain errors

3 protocols using goat anti rabbit or anti mouse immunoglobulin g igg horseradish peroxidase hrp secondary antibodies

1

Evaluating Therapeutic Agents for Kidney Injury

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Trichostatin A (TSA), dimethyl sulfoxide (DMSO), valproate (VPA), and CP were obtained from Sigma Aldrich (St. Louis, MO, USA). CP is 1 mg/ml solution in sterile normal saline. C646 was purchased from Apexbio (USA). β-actin and kidney injury molecule-1 (KIM-1) primary antibodies and goat anti-rabbit or anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP) secondary antibodies were purchased from Bioss (Beijing, China). H3K27Ac antibody was obtained from Abcam (UK). Human recombinant IL-9 was purchased from Bioworld Technology (Nanjing, China). Creatinine (Cr) and blood urea nitrogen (BUN) assay kits were purchased from Njjcbio (Nanjing, China). ELISA kits for detection of IL-9 and TNF-α were purchased from Jymbio (Wuhan, China).
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2

Lysophosphatidylcholine-Induced Cell Signaling

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Lysophosphatidylcholine (Sigma, St. Louis, MO) was dissolved as previously described and used for cell culture studies at a concentration of 20 µM16 (link). β-actin, DR5, kidney injury molecule-1 (KIM-1) primary antibodies and goat anti-rabbit or anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP) secondary antibodies were purchased from Bioss (Beijing, China). Antibodies against TSG101, CD63, ARF6, calnexin, TRAIL, LRG1, and GAPDH were obtained from Abcam (Cambridge, UK). Human recombinant LRG1 and TRAIL were purchased from Abcam (Cambridge, UK). A Cr assay kit was purchased from Njjcbio (Nanjing, China). An ELISA kit for ALB was purchased from Jymbio (Wuhan, China). The Annexin V-FITC/PI Apoptosis Detection Kit was purchased from Bestbio (Shanghai, China). Cytochalasin D was obtained from APExBIO (USA). PKH67 was obtained from Sigma (St. Louis, MO).
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3

Extracellular Vesicle Characterization Protocol

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β-actin, TGFβR1 and goat anti-rabbit or anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP) secondary antibodies were purchased from Bioss (Beijing, China). Antibodies against Tumor susceptibility gene 101 (TSG101), CD63, Adenosine diphosphate ribosylation factor 6 (ARF6), Calnexin, and LRG1 were obtained from Abcam (Cambridge, UK). Cytochalasin D was obtained from APExBIO (USA). PKH67 was obtained from Sigma (St. Louis, MO). A83-01 and LY-364947 were purchased from MCE (USA).
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