The glutaraldehyde-fixed cardiac samples were washed in phosphate buffer three times for 15 minutes each at 4°C, post-fixed in 1% osmium tetroxide solution for 2 hours at 4°C, dehydrated through ascending concentrations of ethyl alcohol, cleared in propylene oxide, embedded in Epon-Araldite mixture (Embed-812; EMS, Fort Washington, PA, USA), and polymerized at 60°C. Semithin sections (1 µm-thick) were cut, stained with 1% toluidine blue, and examined by light microscope to evaluate the histopathological alterations. Ultra-thin sections (50–60 nm thick) were sliced by ultra-microtome (Leica RM 2125; Leica Biosystems Nussloch, GmbH, Germany), picked up on copper grids, stained with 2% uranyl acetate and lead citrate [18 ], and examined using a transmission electron microscope (Jeol TEM 1200 EX; Jeol Ltd., Tokyo, Japan) at 80 Kv.
Tem 1200 ex
Manufactured by JEOL
Sourced in Japan
The JEOL TEM 1200 EX is a transmission electron microscope designed for high-resolution imaging and analysis of materials. It features a maximum accelerating voltage of 120 kV and a resolution of up to 0.34 nm. The TEM 1200 EX is capable of performing various electron microscopy techniques, including bright-field and dark-field imaging, selected-area electron diffraction, and energy-dispersive X-ray spectroscopy.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tem 1200 ex
1
Ultrastructural Analysis of Cardiac Tissue
2
Transmission Electron Microscopy of HNTs-ERY
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The structure of HNTs loaded with ERY was characterized by transmission electron microscopy (Jeol TEM 1200EX, JEOL Ltd., Tokyo, Japan) at an accelerating voltage of 100 kV. The sample was dispersed on a copper grid with carbon support film.
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