Cytokeratin 8

Manufactured by BioLegend

Sourced in United Kingdom

Cytokeratin-8 is a protein that is a member of the cytokeratin family. It is commonly used as a marker for epithelial cells.

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Lab products found in correlation

Antigen unmarking solution, by Vector Laboratories (1 mentions) Keratin 5, by BioLegend (1 mentions) Pannoramic 250 flash 3, by 3DHISTECH (1 mentions) Il 6r, by R&D Systems (1 mentions) Tunel assay kit, by Abcam (1 mentions) Pannoramic viewer software, by 3DHISTECH (1 mentions) Fibronectin, by Merck Group (1 mentions) Phalloidin, by Thermo Fisher Scientific (1 mentions) Vimentin, by Agilent Technologies (1 mentions) Cytokeratin 5, by Abcam (1 mentions) Laminin, by Merck Group (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Agilent Technologies (1 mentions) Alexa conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Recombinant human cxcl12, by Thermo Fisher Scientific (1 mentions) Drebrin, by Abcam (1 mentions) P35 25, by Cell Signaling Technology (1 mentions) Perk1 2 thr202 tyr204 antibody, by Cell Signaling Technology (1 mentions) Matrigel, by Corning (1 mentions) Gapdh, by GeneTex (1 mentions)

2 protocols using cytokeratin 8

Comprehensive Immunohistochemistry Profiling

Cited 1 time

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Immunohistochemistry was performed as previously described (17 (link)). A pressure cooker (95°C for 30 min followed by 120°C for 10 s) was used for antigen retrieval using Antigen Unmarking Solution (Vector Laboratories). Antibodies specific to CHD1 (Sigma, #HPA022236), AKT-473P (Cell Signaling, #4060s), Keratin 5 (BioLegend 905501), Cytokeratin-8 (BioLegend 904801), CD8 (Bioss bs-0648R), Ly6G (BioLegend 127601), CD15 (DAKO M3631), IL‑6R (R&D Systems AF1830-SP) and Ki67 (Thermo Scientific RM 9106-S1) were purchased. TUNEL staining was performed using the TUNEL Assay Kit (Abcam ab206386) according to manufacturer’s instructions. Slides were scanned using Pannoramic 250 Flash III (3DHISTECH Ltd) and images were captured through Pannoramic Viewer software (3DHISTECH Ltd). Human prostate hyperplasia and cancer tissue array samples were purchased from US Biomax (PR753a).

Zhao D., Cai L., Lu X., Liang X., Li J., Chen P., Ittmann M., Shang X., Jiang S., Li H., Meng C., Flores I., Song J.H., Horner J.W., Lan Z., Wu C.J., Li J., Chang Q., Chen K.C., Wang G., Deng P., Spring D.J., Wang Y.A, & DePinho R.A. (2020). Chromatin Regulator, CHD1, Remodels the Immunosuppressive Tumor Microenvironment in PTEN-Deficient Prostate Cancer. Cancer discovery, 10(9), 1374-1387.

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Immunofluorescence Staining of Neuronal Markers

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Cdk5 (Abcam, Cambridge, UK; clone 2G2), cytokeratin 5 (Abcam, EP1601Y), cytokeratin 8 (BioLegend, London, UK; clone 1E8), drebrin (GeneTex, Isleworth, UK; GTX11068), drebrin (Abcam 176318 & 178408), drebrin (Progen, Heidelberg, Germany; clone Mx823), EB1 (BD Transduction Laboratories, Oxford, UK; clone 5), EB3 (Millipore, Consett, UK; AB6033), p35 (Abcam, ab66064), GAPDH (GeneTex, clone GT239), GFP (Abcam, 6556), laminin (Sigma-Aldrich, Poole, UK; clone LAM-89), p35/25 (Cell Signaling, Hitchin, UK; C64B10), p63 (Abcam, ab735 clone BC4A4), pERK1/2 (Thr202/Tyr204) antibody (Cell Signaling; #9101), pS142-drebrin (Millipore, clone 3C14), tyrosinated α-tubulin (SeroTec, Kidlington, UK; clone YL 1/2), vimentin (Dako, Glostrup, Denmark; clone V9). Horseradish peroxidase-conjugated secondary antibodies were from Dako. Alexa-conjugated secondary antibodies and phalloidin were from Life Technologies, Warrington, UK. BTP2 (YM-58483) was from Cambridge Bioscience (Cambridge, UK; CAY13246). Recombinant human CXCL12 was from PeproTech (London, UK; 300-28A). Matrigel was from Corning (New York, USA; 354234) and fibronectin from Millipore.

Dart A.E., Worth D.C., Muir G., Chandra A., Morris J.D., McKee C., Verrill C., Bryant R.J, & Gordon-Weeks P.R. (2017). The drebrin/EB3 pathway drives invasive activity in prostate cancer. Oncogene, 36(29), 4111-4123.

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