HT1080 cells (male, fibrosarcoma epithelial cells purchased from ATCC) were grown at 37 °C with 5% CO2 in RPMI 1640 (Gibco), 10% (v/v) FBS (HyClone), and 1% Penicillin-Streptomycin antibiotic (Corning). A375 cells (female, malignant melanoma; kindly gifted by Oliver Jonas, Koch Institute, MIT), KP cells (female, mouse KrasG12D;p53−/− lung adenocarcinoma; kindly gifted by Tyler Jackson Laboratory), and MEF (Mouse Embryonic Fibroblasts, sex unspecified in the original publication) wild-type (Rev1+/+) and Rev1 knockout (Rev1−/−) cells (Jansen et al., 2006 (link)) were grown at 37 °C with 5% CO2 in DMEM (Gibco), 10% (v/v) FBS (HyClone), and 1% Penicillin-Streptomycin antibiotic (Corning). LNCap cells (male, human prostate adenocarcinoma; kindly gifted by Michael Yaffe Lab, Koch Institute, MIT) were also grown at 37 °C with 5% CO2 in RPMI 1640 (-phenol) (Gibco), 10% (v/v) FBS (HyClone), and 1% Penicillin-Streptomycin antibiotic (Corning). AG01522 cells (male, human primary cells purchased from Coriell Institute) were grown at 37 °C with 5% CO2 in MEM (-Glutamine; +Earle’s Salts; +Non-Essential Amino Acids) (Gibco) and 20% (v/v) FBS (HyClone). All cells were trypsinized using 0.25% Trypsin-EDTA (ThermoFisher) for passaging.
Penicillin streptomycin antibiotic
Manufactured by Corning
Sourced in United States
Penicillin/Streptomycin antibiotics are a combination of two commonly used antibiotics, penicillin and streptomycin. They are used to inhibit the growth of a wide range of bacteria, including both gram-positive and gram-negative species.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Corning (4 mentions)
Hek293t, by American Type Culture Collection (3 mentions)
Fetal bovine serum (fbs), by Merck Group (3 mentions)
Dulbecco modified eagle medium (dmem), by Corning (3 mentions)
Ht1080 cells, by American Type Culture Collection (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium, by Corning (2 mentions)
Trypsin edta, by Corning (2 mentions)
Mccoy s 5a medium, by Merck Group (2 mentions)
H1650, by American Type Culture Collection (2 mentions)
Hek293t, by Corning (2 mentions)
Plasmocin, by InvivoGen (2 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
B 27 serum free supplement, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Corning (1 mentions)
Recombinant human pdgf, by R&D Systems (1 mentions)
Epidermal growth factor (egf), by Merck Group (1 mentions)
Minimum essential medium (mem), by Corning (1 mentions)
Supplementmix, by PromoCell (1 mentions)
12 protocols using penicillin streptomycin antibiotic
1
Culture of Various Cell Lines
2
Culturing Glioblastoma and Endothelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
U87 and U251 (glioblastoma multiforme; GBM) were purchased from the Korea Cell Line Bank (KCLB) and cultured in minimum essential medium (MEM) (Corning, Corning, NY, USA) at 37°C in a humidified atmosphere containing 5% CO2. The medium contained 10% fetal bovine serum and 5% penicillin- streptomycin antibiotics (Corning, Corning, NY, USA). HUVECs were cultured in endothelial cell growth medium MV2 with supplement mix (Promo Cell, Heidelberg, Germany). Two patient-derived GSCs, C2M and X08, were cultured with DMEM-F-12 (Corning, Corning, NY, USA) containing B27 serum-free supplement (Gibco, Carlsbad, CA, USA), epidermal growth factor (EGF) (Sigma-Aldrich, St. Louis, MO, USA), human fibroblastic growth factor (hFGF) (Biovision, Milpitas, CA, USA), and 5% penicillin-streptomycin antibiotics (Corning). Recombinant human PDGF (R&D Systems, Minneapolis, MN, USA) dissolved in 0.4 mM HCl was treated with Opti MEM medium to cells.
3
Cell Culture and Transfection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HEK293T and B16-OVA (B16F10 cells expressing OVA) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM; CORNING) supplemented with 10% fetal bovine serum (FBS) (CORNING) and 1% penicillin/streptomycin antibiotics (CORNING). U2OS cells were maintained in McCoy’s 5A medium (CORNING) containing 10% FBS and 1% penicillin/streptomycin antibiotics. Cell transfection was carried out by Lipofectamine 2000 according to the manufacturer’s protocol (Life Technologies).
4
Cell Culture Conditions for HT1080, MEFs, and KP Cells
5
Generating 5-FU and Oxaliplatin Resistant Colorectal Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HT29 cells (female, colorectal adenocarcinoma cells purchased from ATCC) were grown at 37 °C with 5% CO2 in McCoy's 5a medium (SigmaAldrich), 10% (v/v) FBS (Gibco), and 1% Penicillin–Streptomycin antibiotic (Corning). SW480 cells (male, colorectal adenocarcinoma cells purchased from ATCC) were grown at 37 °C with 5% CO2 in L-15 medium (SigmaAldrich), 10% (v/v) FBS (Gibco), and 1% Penicillin–Streptomycin antibiotic (Corning). 5-FU resistant cells were generated by incubating its parental cells with 5-FU at 0.5 µM, 1 µM, 2 µM and 4 µM for 2 weeks (48 h treatment with incubation in drug free medium for 12 days). Oxaliplatin resistant cells were generated by incubating its parental cells with oxaliplatin at 2 µM, 4 µM, 8 µM and 16 µM for 2 weeks (48 h treatment with incubation in drug free medium for 12 days). The resistant cells were kept in the medium with drugs for at least another 16 weeks. Entire population of resistant cells were used in this study.
6
Human Cell Line Maintenance Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human NSCLC cell lines A549, H1650 and H226 as well as the transformed human embryonic kidney epithelial cell line, HEK-293T, were purchased from American Type Culture Collection and maintained according to the manufacturer’s instructions. HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium (Corning) and lung cancer cell lines were cultured in RPMI-1640 medium (Corning). Media were supplemented with 10% fetal bovine serum (Millipore) and 1% Penicillin/Streptomycin antibiotics (Corning). All cell lines were certified by the indicated cell bank and routinely authenticated by morphologic inspection.
7
Culturing Human Mammary and Breast Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human mammary epithelial cells (MCF10A) were obtained from American Type Culture Collection (Manassas, VA, USA). Breast cancer cell lines (ZR-75-1, MCF-7, BT-474, MDA-MB-453, HCC1937, HCC38, and MDA-MB-231) were obtained from the Korea Cell Line Bank (KCLB). MCF10A cells are cultured in Dulbecco’s modified Eagle’s medium (DMEM)/F12 media (Thermo Fisher Scientific, Invitrogen, USA) supplemented with 100 ng/mL cholera toxin, 20 ng/mL EGF, 0.01 mg/mL insulin, 500 ng/mL hydrocortisone, and 5% Chelex-treated horse serum. ZR-75-1, BT-474, MDA-MB-453, and MDA-MB-231 cells were maintained in DMEM (Corning, Corning, NY, USA), and MCF-7, HCC1937, and HCC38 cells were maintained in RPMI (Corning, Corning, NY, USA). HUVECs were grown in endothelial cell growth medium MV2 with supplement mix (Promo Cell, Heidelberg, Germany). All medium was supplemented with 10% fetal bovine serum (FBS; Tissue Culture Biologicals, Tulare, CA, USA) and 1% penicillin-streptomycin antibiotics (Corning, Corning, NY, USA). Cells were maintained in a humidified incubator of 5% CO2 at 37°C.
8
NSCLC Cell Lines and Gefitinib Sensitivity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
gefitinib-sensitive HCC827 parental (HCC827P) and HCC827 gefitinib-resistant (HCC827GR) human NSCLC cell lines were a generous gift from Dr. Pasi Jänne [52 (link)]. gefitinib-sensitive PC9 parental (PC9P) cells and their gefitinib-resistant counterparts (PC9GR2, PC9GR3) were kindly provided by Dr. Aaron Hata [53 (link)]. HCC827P and PC9P cells were grown in RPMI-1640 medium (Corning). RPMI-1640 medium containing 1 µm of gefitinib (Selleckchem) was used to culture HCC827GR, PC9GR2, and PC9GR3 cells. HEK-293T cells were purchased from ATCC and maintained in DMEM (Corning). All media were supplemented with 10% fetal bovine serum (FBS; Millipore), 1% penicillin/streptomycin antibiotics (Corning), and 25 µg/ml Plasmocin prophylactic (Invivogen). All cell lines were authenticated by their source and were subsequently routinely authenticated via morphologic inspection.
9
Cultivation of Human SCLC Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human SCLC cell lines, DMS-53 and H1048, were purchased from The European Collection of Authenticated Cell Cultures (ECACC, Salisbury, UK) via Sigma-Aldrich (St. Louis, MO) and American Type Culture Collection (ATCC, Manassas, VA), respectively, and maintained in RPMI-1640 medium (Corning, Manassas, VA) supplemented with 10% foetal bovine serum (FBS; Millipore, Burlington, MA) and antibiotics. Human 293T cells obtained from ATCC were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Corning). All cells were grown in a humidified chamber at 37 °C supplied with 5% CO2 in media supplemented with 10% FBS, 1% Penicillin/Streptomycin antibiotics (Corning), and 25 µg/mL plasmocin (Invivogen, San Diego, CA). All cell lines were certified prior to purchase and were subsequently authenticated by morphologic inspection on a regular basis.
10
Cell Line Cultivation and Maintenance
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!