The middle 4 cm of the small and large intestine, respectively, the mesenteric lymph nodes (MLN), and the spleen were collected from each mouse. Tissues were fixed in phosphate buffered saline (PBS) with 4% paraformaldehyde for 20 hours at room temperature (RT), dehydrated through a series of immersions in graded ethanol, and embedded in paraffin. The tissue blocks were sectioned (thickness 4 μm) using a microtome (Thermo Fisher Scientific) and slides placed onto a waterbath (40°C) for 2 minutes to reduce wrinkles. Sections were transferred to positively charged slides (Dako FLEX IHC Microscope Slides, Dako), and left to dry (24 hours at RT). Slides were heated in an oven at 60°C for 30 minutes, followed by deparaffinization with xylene and then rehydration. Antigen retrieval was performed by incubations in either high or low pH heat-induced epitope retrieval solution (HIER; DAKO-DM829, Dako) in a HIER apparatus (PT-link 200, Dako). Slides were allowed to cool at room temperature for 30 minutes, followed by a wash in PBS.
Dako flex ihc microscope slides
Manufactured by Agilent Technologies
The Dako FLEX IHC Microscope Slides are a specialized product designed for immunohistochemistry (IHC) applications. These slides provide a surface for mounting and analyzing tissue samples using IHC techniques.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dako flex ihc microscope slides
1
Tissue Preparation for Histological Analysis
2
PD-L1 IHC 22C3 Staining Protocol
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A specimen is defined as an FFPE tumor tissue block. Sections were cut at 4 µm thickness, placed on Dako FLEX IHC Microscope Slides (Code K8020; Dako North America Inc., Carpinteria, CA) or Superfrost Plus charged glass slides, and oven-dried at 58±2°C for ~1 hour. To preserve the PD-L1 antigen on mounted tissue sections, the cut sections were stored in the dark at 2 to 8°C before immunostaining with PD-L1 IHC 22C3 pharmDx (code SK006; Dako North America Inc.). The slides were stained within 1 month of sectioning or within the timeframe described in the PD-L1 IHC 22C3 pharmDx package insert22 for the relevant tumor types.
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