Wyatt minidawn treos mals instrument

Size-exclusion chromatography with multi-angle light scattering (SEC-MALS) experiments for XRCC6 (aa 1-609) and the FH domain of FOXL2 (aa 46-158) were performed using FPLC system (GE Healthcare) connected to a Wyatt MiniDAWN TREOS MALS instrument and a Wyatt Optilab rEX differential refractometer (Wyatt Technology, Santa Barbara, CA, USA). A Superdex 200 10/300 GL (GE Healthcare) gel filtration column pre-equilibrated with buffer A, was normalised using ovalbumin. Proteins were injected at a flow rate of 0.4 ml/min. Data were analysed using the Zimm model for static light-scattering data fitting, and graphs were constructed using EASI graph with a UV peak in the ASTRA V software (Wyatt Technology).

SEC-MALS was implemented with an FPLC machine (GE Healthcare, Chicago, IL, USA) connected to a Wyatt MiniDAWN TREOS MALS instrument and a Wyatt Optilab rEX differential refractometer (Wyatt Technology, Santa Barbara, CA, USA). A HiLoad^™ 10/300 Superdex 200 GL (GE Healthcare, Chicago, IL, USA) column was pre-equilibrated with a buffer containing 20 mM Tris-HCl pH 7.5, 150 mM sodium chloride, and 0.5 mM TCEP, and was normalized using ovalbumin. 100 μL of monomer and dimer NDRG3 ΔNC at 2.0 mg/mL were injected into the machine at flow rate of 0.4 mL/min, respectively. Data were analyzed using the Zimm model for fitting static light-scattering data and graphed using EASI graph with a UV peak in the ASTRA V software (Wyatt Technology, Santa Barbara, CA, USA).