Se 61 ri detector

The GPC experiments for the samples studied were conducted in a chloroform solution at 35 °C and at a flow rate of 1 mL/min using a Viscotek VE 1122 (Malvern, Worcestershire, UK) pump with two Mixed C PLgel styragel columns (Agilent, Santa Clara, CA, USA) in series and a Shodex SE 61 RI detector (Showa Denko, Munich, Germany). A volume of 10 μL of a chloroform sample solution (concentration 0.5% m/V) was injected into the system. The instrument was calibrated using polystyrene standards with a low dispersity.

The molar mass and molar-mass dispersity of the samples were determined using gel permeation chromatography conducted in chloroform solution at 35 °C with an eluent flow rate of 1 mL/min using a Viscotek VE 1122 (Malvern, UK) pump with two Mixed C PLgel styragel columns (Agilent, Santa Clara, CA, USA) in series and a Shodex SE 61 RI detector (Showa Denko, Munich, Germany). Polystyrene standards (Calibration Kit S-M-10, Polymer Laboratories) with narrow molar-mass dispersity were used to generate a universal calibration curve. The samples were measured using OmniSEC 5.0 (Viscotek, Malvern, UK) software. The molar mass loss was calculated using the equation:
where M_w0 is the initial mass-average molar mass and M_wx is the consecutive or final average molar mass.

The number-average molar mass (M_n) and the molar mass distribution index (M_w/M_n) of the obtained PHA samples were determined by GPC experiments conducted in a chloroform solution at 35 °C and at a flow rate of 1 mL/min using a Viscotek VE 1122 (Malvern, Worcestershire, UK) pump with two Mixed C PLgel Styragel columns (Agilent, Santa Clara, CA, USA) in series, and a Shodex SE 61 RI detector (Showa Denko, Munich, Germany). A volume of 10 μL of a chloroform sample solution (concentration 0.5% m/V) was injected into the system. The instrument was calibrated using polystyrene standards with low dispersity.