HuH1, HuH7, HLE, HLF, Hep3B, HEP-G2, SK-Hep-1, and PLC/PRL/5 human liver cancer cell lines were obtained from the Japanese Collection of Research Bioresources (JCRB; Osaka, Japan) or American Type Culture Collection (ATCC; Manassas, VA). Cells were routinely cultured in DMEM supplemented with 10% FBS. Two fresh HCC specimens (HCC1 and HCC2) were obtained and were used for xenotransplantation and to prepare single-cell suspensions for analysis. Primary HCC tissues were dissected and digested in 1 mg/mL type 4 collagenase (Sigma-Aldrich Japan K.K., Tokyo, Japan) solution at 37 °C for 15–30 min. Contaminated red blood cells were lysed with ammonium chloride solution (STEMCELL Technologies, Vancouver, BC, Canada) on ice for 5 min.
Sk hep 1
Manufactured by Japanese Collection of Research Bioresources Cell Bank
Sourced in Japan
SK-Hep-1 is a human liver adenocarcinoma cell line. It is a commonly used cell line in cancer research and drug discovery.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by Japanese Collection of Research Bioresources Cell Bank (4 mentions)
Hep3b, by Japanese Collection of Research Bioresources Cell Bank (4 mentions)
K02288, by Selleck Chemicals (2 mentions)
Ldn 212854, by Selleck Chemicals (2 mentions)
Hek293t, by Japanese Collection of Research Bioresources Cell Bank (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Plc prf 5, by RIKEN Cell Bank (1 mentions)
Hepg2, by RIKEN Cell Bank (1 mentions)
Dulbecco s modified eagle s medium dmem, by Merck Group (1 mentions)
Williams e medium, by Merck Group (1 mentions)
Effectene transfection reagent, by Qiagen (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Human umbilical vein endothelial cells (huvec), by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Egm 2 bulletkit, by Lonza (1 mentions)
Nci h460, by American Type Culture Collection (1 mentions)
Mcf 7, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Bxpc 3, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Hep3b, by American Type Culture Collection (1 mentions)
Plc prf 5, by American Type Culture Collection (1 mentions)
6 protocols using sk hep 1
1
Establishment and Characterization of Human Liver Cancer Cell Lines
2
Establishment of Diverse Hepatoma Cell Lines
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The human hepatoma cell lines Huh7, Hep3B, HLE, JHH7 and SkHep1 were obtained from the Japanese Collection of Research Bioresources (Osaka, Japan). HepG2 and PLC/PRF/5 were obtained from RIKEN Cell Bank (Tsukuba, Japan) and the embryonic kidney cell line 293 T was from the American Type Culture Collection (Manassas, VA, USA)
JHH7 was maintained in William's E Medium (Sigma, St Louis, MO, USA) containing 10% heat-inactivated fetal bovine serum (FBS). Other cell cultures were maintained in Dulbecco's modified Eagle's medium (DMEM; Sigma) containing 10% heat-inactivated FBS. The cells were transfected using the Effectene Transfection Reagent (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
JHH7 was maintained in William's E Medium (Sigma, St Louis, MO, USA) containing 10% heat-inactivated fetal bovine serum (FBS). Other cell cultures were maintained in Dulbecco's modified Eagle's medium (DMEM; Sigma) containing 10% heat-inactivated FBS. The cells were transfected using the Effectene Transfection Reagent (Qiagen, Hilden, Germany) according to the manufacturer's instructions.
3
Comparative Analysis of HCC Cell Lines
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The HCC cell lines Huh7, HepG2, HLE, HLF, and SK-Hep-1 and HUVEC were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan), American Type Culture Collection (Manassas, VA, USA) or Procell Life Science & Technology (Wuhan, China). The HCC cell line MT was established from resected HCC specimens as described previously [17 (link)]. HCC cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with foetal bovine serum (FBS; Gibco) at 37 °C. HUVECs were maintained in an endothelial cell growth medium-2 (EGM-2 BulletKit; Lonza, Basel, Switzerland). The recombinant BMP9 protein was purchased from R&D Systems (Minneapolis, MN, USA) and Abcam (Cambridge, UK). The BMP receptor inhibitors K02288 and LDN-212854 were purchased from Selleck Chemicals (Houston, TX, USA) and dissolved in dimethyl sulfoxide (Sigma Aldrich, St. Louis, MO, USA).
4
Cell Line Culture Methodology
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HEK-293T, NCI-H1299, BxPC-3, PANC-1, Hep3B, PLC/PRF/5,HepG2, SK-HEP-1, MCF7, A549, NCI-H460, Tera-1 and Tera-2 were purchased from ATCC; HuH-7 was purchased from Japanese Collection of Research Bioresources (JCRB), SMMC-7721 and BEL-7402 were purchased from Typical culture preservation commission cell bank, Chinese academy of sciences (NCB); MHCC-97L and LM3 were gifts from Zhongshan Hospital, Fudan University (Shanghai, China); SMMC-7721, BEL-7402, MHCC-97L and LM3 used in this study have been described in previous publication [24] (link), [30] (link), [31] , [32] (link).
HEK-293T, NCI-H1299, BxPC3, PANC1, Hep3B, PLC/PRF/5, HepG2, HuH-7, HepG2, SK-HEP-1, SMMC-7721, 97L, LM3, BEL-7402 and MCF7 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum(FBS). A549 and NCI-H460 cells were cultured in RPMI1640 medium supplemented with 10% FBS. Tera1 and Tera2 cells were cultured in McCoy’s 5a medium supplemented with 15%FBS. All cell lines were cultured in the presence of antibiotics at 37°C with 5%CO2.
HEK-293T, NCI-H1299, BxPC3, PANC1, Hep3B, PLC/PRF/5, HepG2, HuH-7, HepG2, SK-HEP-1, SMMC-7721, 97L, LM3, BEL-7402 and MCF7 cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum(FBS). A549 and NCI-H460 cells were cultured in RPMI1640 medium supplemented with 10% FBS. Tera1 and Tera2 cells were cultured in McCoy’s 5a medium supplemented with 15%FBS. All cell lines were cultured in the presence of antibiotics at 37°C with 5%CO2.
5
Comparative Analysis of HCC Cell Lines
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The HCC cell lines Huh7, Huh1, HLE, HLF, and SK‐Hep‐1 were obtained from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan) or the American Type Culture Collection (Manassas, VA, USA). The HCC cell line MT was established from resected HCC specimens as described previously [11 (link)]. The cells were maintained in Dulbecco's modified Eagle medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% FBS (Gibco) at 37 °C. The BMP receptor inhibitors K02288 and LDN‐212854 and the TGF‐β receptor inhibitor galunisertib were purchased from Selleck Chemicals (Houston, TX, USA) and dissolved in dimethyl sulfoxide. The selectivity of the inhibitors is shown in Table S1 .
6
Cell Line Culture Protocols
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Human embryonic kidney cell line HEK-293T, human HCC cell lines Huh-7, HepG2, Hep3B and SK-HEP1 and normal liver cell line HL-7702 were purchased from the Japanese Collection of Research Bioresources (Tokyo, Japan). The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco BRL, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS) (Gibco) and incubated at 37°C in a humidified chamber containing 5% CO2.
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