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Cd8α pe cy7

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The CD8α-PE/Cy7 is a fluorescently-labeled monoclonal antibody used for the detection and quantification of CD8α-positive cells in flow cytometry applications. It is composed of a phycoerythrin (PE) fluorescent dye conjugated to a cyanine 7 (Cy7) tandem dye, providing a bright signal and good spectral separation from other commonly used fluorochromes.

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Lab products found in correlation

Facscanto 2, by BD (2 mentions) Siglecf pe, by BD (2 mentions) Cd40 pe, by BD (2 mentions) Foxp3 staining kit, by Thermo Fisher Scientific (2 mentions) Gr1 fitc, by BD (2 mentions) Livedead aqua or blue, by Thermo Fisher Scientific (2 mentions) Cd4 apc or a700, by BD (2 mentions) Cd19 e780, by BD (2 mentions) Cd44 bv570, by BD (2 mentions) Cd69 fitc cd86 af488, by BD (2 mentions) Foxp3 e450, by BD (2 mentions) Hucd2 pe, by BD (2 mentions) Igm apce780, by BD (2 mentions) Tcrβ apce780, by BD (2 mentions) Duosets, by Thermo Fisher Scientific (2 mentions) Cd80 percp cy5, by BD (2 mentions) F4 80 pe cy7, by BD (2 mentions) Cd11b bv711, by BD (2 mentions) Cd3 apce780, by BD (2 mentions) Facs canto 2 instrument, by BD (1 mentions)

Close spelling variants of this product

CD8α-PE (7 citations) PE-Cy7-anti-CD8α (2 citations)

3 protocols using cd8α pe cy7

1

Multiparameter Flow Cytometry Immunophenotyping

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Fc-gamma receptors were blocked with a rat anti-mouse CD16/CD32 antibody (BD). Mouse-specific antibodies were the following: CD3-Brilliant violet 510, CD25-PercP Cy5.5, I-A/I-E-Brilliant violet 510, CD45R/B220-PE-Cy7, CD43-APC, CTLA-4-APC (all from BioLegend), CD4-APC-Cy7, CD69-PE, CD11c-PercP Cy5.5, CD19-APC-Cy7, CD40-FITC (all from BD Pharmingen), CD8α-PE-Cy7, FoxP3-FITC, CD86-APC, CD83-FITC, CD80-PE-Cy7, IgM-PercP-eFluor 710 (all from eBioscience).
During the experimental set-up, CD3 antibody was included in the staining panel (BV510 BioLegend clone 17A2) and used for the gating of T cells. The results obtained with gating on CD3+ CD4+ CD8- were similar to those obtained with gating on CD4+ CD8-. Due to the limitation in the maximum number of colours that can be discriminated with the FACS Canto II, CD3 staining was omitted in subsequent stainings.
Dead cells were excluded using the fixable viability dye-eFluor 450 (eBioscience), and intracellular staining was performed using the fixation/ permeabilization buffer set from eBioscience. Flow cytometry measurements were performed on a FACS Canto II instrument (BD) and the data were analyzed with FlowJo software (Tree Star).
Sordé L., Spindeldreher S., Palmer E, & Karle A. (2017). Massive immune response against IVIg interferes with response against other antigens in mice: A new mode of action?. PLoS ONE, 12(10), e0186046.
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2

Multiparametric Flow Cytometry Analysis

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Cells were first stained with LiveDead aqua or blue (Life Technologies). Following FcR-Block (2.4G2), cells were stained using the following monoclonal antibodies (all used at 1:200 dilution): CD3-APCe780, CD4-APC or A700, CD8α-PE/Cy7, CD11c-APC or BV421, MHC-II-PerCP/Cy5.5 or eFlour450, CD11b-BV711 or PE, CD19-e780, CD80-PerCP/Cy5.5, CD40-PE, CD44-BV570, CD69-FITC CD86-AF488, Foxp3-e450, F4/80-PE/Cy7, Gr1-FITC, HuCD2-PE, IgM-APCe780, SiglecF-PE and TCRβ-APCe780 (BD Biosciences, BioLegend or eBioscience). Foxp3 staining was performed with the eBioscience FoxP3 staining kit. Samples were acquired using FACS LSR II or FACS Canto II using BD FACSDiva software and analysed with FlowJo v.9 software (Tree Star). Cytokines were measured in culture supernatants by ELISA using paired monoclonal antibody, and recombinant cytokine standards, or Duosets (eBioscience, BD Biosciences, BioLegend, R&D Systems and Peprotech).
Cook P.C., Owen H., Deaton A.M., Borger J.G., Brown S.L., Clouaire T., Jones G.R., Jones L.H., Lundie R.J., Marley A.K., Morrison V.L., Phythian-Adams A.T., Wachter E., Webb L.M., Sutherland T.E., Thomas G.D., Grainger J.R., Selfridge J., McKenzie A.N., Allen J.E., Fagerholm S.C., Maizels R.M., Ivens A.C., Bird A, & MacDonald A.S. (2015). A dominant role for the methyl-CpG-binding protein Mbd2 in controlling Th2 induction by dendritic cells. Nature Communications, 6, 6920.
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3

Flow Cytometry Immunophenotyping Panel

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Cells were first stained with LiveDead aqua or blue (Life Technologies). Following FcR-Block (2.4G2), cells were stained using the following mAb (all used at 1:200 dilution): CD3-APCe780, CD4- APC or A700, CD8α-PE/Cy7, CD11c- APC or BV421, MHCII-PerCP/Cy5.5 or eFlour450, CD11b-BV711 or PE, CD19-e780, CD80-PerCP/Cy5.5, CD40-PE, CD44-BV570, CD69-FITC CD86-AF488, Foxp3-e450, F4/80-PE/Cy7, Gr1-FITC, HuCD2-PE, IgM-APCe780, SiglecF-PE and TCRβ-APCe780 (BD Biosciences, BioLegend, or eBioscience). Foxp3 staining was performed with the eBioscience FoxP3 staining kit. Samples were acquired using FACS LSR II or FACS Canto II using BD FACSDiva software and analyzed with FlowJo v.9 software (Tree Star). Cytokines were measured in culture supernatants by ELISA using paired mAb, and recombinant cytokine standards, or Duosets (eBioscience, BD Biosciences, BioLegend, R&D Systems, and Peprotech).
Cook P.C., Owen H., Deaton A.M., Borger J.G., Brown S.L., Clouaire T., Jones G.R., Jones L.H., Lundie R.J., Marley A.K., Morrison V.L., Phythian-Adams A.T., Wachter E., Webb L.M., Sutherland T.E., Thomas G.D., Grainger J.R., Selfridge J., McKenzie A.N., Allen J.E., Fagerholm S.C., Maizels R.M., Ivens A.C., Bird A, & MacDonald A.S. (2015). A dominant role for the methyl-CpG-binding protein Mbd2 in controlling Th2 induction by dendritic cells. Nature Communications, 6, 6920.
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