Anti c ebpβ sc150x

Manufactured by Santa Cruz Biotechnology

Sourced in China, United States

Anti-C/EBPβ (sc-150X) is a primary antibody product offered by Santa Cruz Biotechnology. It is designed to detect the C/EBPβ (CCAAT/enhancer-binding protein beta) protein in various experimental applications.

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Lab products found in correlation

Ab4729, by Abcam (1 mentions) Anti akt, by Bioworld Technology (1 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Ab10812, by Abcam (1 mentions) Anti glut4, by Santa Cruz Biotechnology (1 mentions) Anti pi3k p85α, by Bioworld Technology (1 mentions) Anti pol 2, by Merck Group (1 mentions) Anti h3k4me3, by Merck Group (1 mentions) Anti h3k4me1, by Abcam (1 mentions) Anti c ebpα sc 61x, by Santa Cruz Biotechnology (1 mentions) Anti pparγ sc 7196x, by Santa Cruz Biotechnology (1 mentions) Anti h3k4me2, by Abcam (1 mentions) Anti h3k27me3, by Merck Group (1 mentions) Anti ctcf, by Merck Group (1 mentions) Anti h3k36me3, by Abcam (1 mentions) Anti p300 sc 585x, by Santa Cruz Biotechnology (1 mentions) Anti h3k9me2, by Abcam (1 mentions) Anti h3k27ac ab4729, by Abcam (1 mentions)

Close spelling variants of this product

C/EBPβ (65 citations) Anti-C/EBPβ (41 citations)

2 protocols using anti c ebpβ sc150x

Protein Expression Analysis of Gastrocnemius Muscle

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Total cellular protein was extracted from 100 mg of frozen gastrocnemius muscle (n = 6 per group) samples as described previously [29 (link)]. Protein concentrations were measured with a Pierce BCA Protein Assay kit (no. 23225, Thermo Scientific). Western blot analysis of target proteins was carried out according to the protocols provided by the manufacturer. The sources of primary antibodies used in Western blot were as follows: anti- PPARγ (AP0686, Bioworld Technology, Nanjing, China), anti-C/EBPβ (sc150X, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-phosphor-ser⁸⁵⁵ HSL (P-HSL, BS4234, Bioworld Technology); anti-HSL (BS2742, Bioworld Technology); anti-ATGL (BS7989, Bioworld Technology); anti- GLUT4 (sc53566, Santa Cruz Biotechnology); anti- phosphor-ser¹¹⁰¹ IRS (BS4239P, Bioworld Technology); anti-IRS (BS3589, Bioworld Technology); anti-PI3K p85α (BS3678, Bioworld Technology); anti-AKT (AP0059, Bioworld Technology); anti-PKCθ (BS3666, Bioworld Technology); anti-Histone 3acetyl K9 (ab10812, abcam, Cambridge, UK); anti-Histone 3acetyl K27 (ab4729, abcam); β-actin (KC5AO8, Kangcheng, Nanjing, China).

Huang Y., Gao S., Chen J., Albrecht E., Zhao R, & Yang X. (2016). Maternal butyrate supplementation induces insulin resistance associated with enhanced intramuscular fat deposition in the offspring. Oncotarget, 8(8), 13073-13084.

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Antibody Characterization for Epigenetic Research

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The following antibodies were used in this study. Anti-C/EBPα (sc-61X, lot: G1112), anti-C/EBPβ (sc-150X, lot: L2910), anti-PPARγ (sc-7196X, lot: H1512), anti-p300 (sc-585X, lot: E2209) were from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-CBP (7389S, lot: 1) was from Cell Signaling Technology (Danvers, MA, USA). Anti-MED1/TRAP220 (A300-793A, lot: 2), anti-RbBP5 (AA300-109A), anti-Menin (A300-105A) were from Bethyl Laboratories (Montgomery, TX, USA). Anti-EBF2 (AF7006, lot: CEUA0113081) was from R&D Systems (Minneapolis, MN, USA). Anti-CTCF (07-729, lot: 2054523), Anti-Pol II (17-672, lot: 2015592), anti-H3K4me3 (07-473, lot: 1974075) and Anti-H3K27me3 (07-449, lot: DAM1387952) were from Millipore (Billerica, MA, USA). Anti-H3K4me1 (ab8895, lot: GR61280-1), anti-H3K4me2 (ab7766, lot: GR102810-1), anti-H3K9me2 (ab1220, lot: 957002), anti-H3K27ac (ab4729, lot: GR81163-1), anti-H3K36me3 (ab9050, lot: 947436) were from Abcam (Cambridge, MA, USA). Anti-UTX was described previously (21 (link)). Anti-MLL4 and anti-SET1A were described previously (22 ).

Lai B., Lee J.E., Jang Y., Wang L., Peng W, & Ge K. (2017). MLL3/MLL4 are required for CBP/p300 binding on enhancers and super-enhancer formation in brown adipogenesis. Nucleic Acids Research, 45(11), 6388-6403.

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