Pti quantamaster

Manufactured by Horiba

The PTI QuantaMaster is a spectrofluorometer designed for the measurement of fluorescence and phosphorescence. It provides high-sensitivity detection and is capable of performing steady-state and time-resolved fluorescence measurements.

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QuantaMaster (20 citations) PTI QuantaMaster 400 (6 citations) PTI QuantaMaster Fluorimeter (5 citations) PTI QuantaMaster QM-4SE (2 citations) PTI QuantaMaster spectrofluorometer (2 citations) PTI QuantaMaster-400 fluorometer (2 citations) PTI QuantaMaster 800 (2 citations) PTI QuantaMaster 400 spectrofluorometer (2 citations) PTI Quantamaster fluorometer (2 citations)

4 protocols using pti quantamaster

Steady-state Fluorescence Measurements

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Steady-state
fluorescence measurements were performed on a PTI QuantaMaster spectrofluorimeter
(Photon Technology International, Lawrenceville, NJ). Sample excitation
was set at 500 nm. Emission was recorded between 507 and 675 nm to
encompass the spectra of both fluorophores. To avoid inner filter
effects, the concentration of the fluorophores was such that the optical
density at the excitation wavelength was OD_l/2 < 0.05. Experiments were performed under constant stirring.
The temperature was controlled by a Peltier element.

Palacios-Ortega J., Rivera-de-Torre E., García-Linares S., Gavilanes J.G., Martínez-del-Pozo Á, & Slotte J.P. (2021). Oligomerization of Sticholysins from Förster Resonance Energy Transfer. Biochemistry, 60(4), 314-323.

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Fluorescence-based Prodrug Characterization

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For all prodrugs, 0.625 μL of a 4 mM pyrene stock prepared in methanol was added to 1 mL of aqueous solution of prodrug at the specified concentration. Samples were then mixed by pipetting and transferred to quartz cuvettes with 5-mm path length. Emission spectra from 350 nm to 450 nm was recorded at 334 nm excitation on a Photon Technology International PTI QuantaMaster fluorescence/luminescence spectrometer. Excitation and emission slit widths were set to 1 nm. All data were collected from triplicate runs. The average ratio between emission peak intensity at 372 nm and 384 nm was then determined and plotted as a function of prodrug concentration.

DeFrates K.G., Engström J., Sarma N.A., Umar A., Shin J., Cheng J., Xie W., Pochan D., Omar A.K, & Messersmith P.B. (2022). The influence of molecular design on structure–property relationships of a supramolecular polymer prodrug. Proceedings of the National Academy of Sciences of the United States of America, 119(44), e2208593119.

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Fluorescent Anisotropy Assay for Factor Xa Binding

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Fluorescent anisotropy measurements were performed with Alexa-488 FXa at 25°C using a PTI QuantaMaster fluorescence spectrophotometer fitted with polarizing lenses (Photon Technology International, Birmingham, NJ). The machine settings were as follows for slit A: 1.4 mm, 0.93 mm, high volt at 850, gain at 10^-3. Slit B was 1.5 nm, high volt at 790, gain at 10^-1. All four cuvettes were utilized in the experimental set up with the following conditions in selection buffer: PCPS, buffer PCPS + Alexa-488 FXa, PCPS + Alexa-488 FXa + T18.3, PCPS + Alexa-488 FXa + Mut1. The final concentrations were 10 μM for PCPS, 100 nM for Alexa-488 FXa, 2.0 μM for T18.3 or Mut1, and FVa was assayed from 0-200 nM. Scattering intensity and errors for each data point were recorded.

Soule E.E., Yu H., Olson L., Naqvi I., Kumar S., Krishnaswamy S, & Sullenger B.A. (2022). Generation of an anticoagulant aptamer that targets factor V/Va and disrupts the FVa-membrane interaction in normal and COVID-19 patient samples. Cell Chemical Biology, 29(2), 215-225.e5.

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Exosome Membrane Fluidity Analysis

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The exosome pellet was re-suspended in 1mL of PBS and labeled with 0.6μM of the membrane probe, diphenyl hexatriene (DPH) (Invitrogen). Fluorescence anisotropy experiments were conducted on a PTI Quantamaster fluorescence spectrophotometer (Photon Technology International), fitted with a Peltier unit. The sample was excited at 355 nm and the emission monitored at 430 nm. Fluorescence polarization and anisotropy were calculated as described previously (24 (link)). The phase behavior and transition was monitored using fluorescence anisotropy as a function of temperature over a temperature range of 4°C to 50°C.

Shenoy G.N., Loyall J., Maguire O., Iyer V., Kelleher RJ J.r., Minderman H., Wallace P.K., Odunsi K., Balu-Iyer S.V, & Bankert R.B. (2018). Exosomes associated with human ovarian tumors harbor a reversible checkpoint of T cell responses. Cancer immunology research, 6(2), 236-247.

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