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Gelvatol

Manufactured by Thermo Fisher Scientific
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Gelvatol is a water-soluble polymer used as a mounting medium for microscopy samples. It can be used to embed and preserve biological specimens for microscopic analysis.

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Lab products found in correlation

Lsm 700, by Zeiss (2 mentions) Prolong diamond, by Thermo Fisher Scientific (2 mentions) Centrin, by Merck Group (2 mentions) Elyra ps 1, by Zeiss (2 mentions) Vectashield, by Vector Laboratories (2 mentions) β actin, by Proteintech (2 mentions) Par6α, by Abcam (2 mentions) Ninein, by BioLegend (2 mentions)

2 protocols using gelvatol

1

Immunofluorescence Staining of Cellular Components

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in ice-cold methanol for 3 min, permeabilized in
blocking buffer (2.5% BSA or FBS, 0.1% TritonX-100,
0.03% NaN3 in DPBS). Primary and secondary antibodies were
diluted in blocking buffer and incubated for 2 h at RT. Coverslips were mounted
using Gelvatol, or Prolong Diamond (ThermoFisher Scientific) and imaged with the
inverted confocal microscope Zeiss LSM700. Images were processed with
ImageJ/FIJI. For 3D-structured illumination microscopy (SIM) (Fig. 1e), WT and KO FEFs were plated on 1.5-mm
coverslips and immunostained as above. Coverslips were mounted with Vectashield
(Vectorlabs). 3D-SIM imaging was performed on a Zeiss Elyra PS.1 microscope
equipped with a 100x/1.40 oil objective lens. Exciting light was directed
through a movable optical grating to generate a fine-striped interference
pattern on the same plane. Z-stacks of 15 optical sections with a step size of
0.1 μm were acquired to generate images in maximum intensity projection.
The epitope of the ASPM (216-1) antibody43 (link), NDNYGLNQDLESES, is located prior to the TALEN target
site. The following antibodies were used at 1:100 – 1:2000: Centrin
(Millipore 20H5), Par6α (SCBT sc-14405), Par6α (Abcam ab180159),
β-actin (Proteintech 20536-1-AP), ASPM (SCBT sc-98903), ASPM (gift from
J. Bond, 216-1), Ninein (Biolegend Poly6028), aPKCζ (SCBT sc-216).
Johnson M.B., Sun X., Kodani A., Borges-Monroy R., Girskis K.M., Ryu S.C., Wang P.P., Patel K., Gonzalez D.M., Woo Y.M., Yan Z., Liang B., Smith R.S., Chatterjee M., Coman D., Papademetris X., Staib L.H., Hyder F., Mandeville J.B., Grant P.E., Im K., Kwak H., Engelhardt J.F., Walsh C.A, & Bae B.I. (2018). Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. Nature, 556(7701), 370-375.
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2

Immunofluorescence Staining of Cellular Components

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in ice-cold methanol for 3 min, permeabilized in
blocking buffer (2.5% BSA or FBS, 0.1% TritonX-100,
0.03% NaN3 in DPBS). Primary and secondary antibodies were
diluted in blocking buffer and incubated for 2 h at RT. Coverslips were mounted
using Gelvatol, or Prolong Diamond (ThermoFisher Scientific) and imaged with the
inverted confocal microscope Zeiss LSM700. Images were processed with
ImageJ/FIJI. For 3D-structured illumination microscopy (SIM) (Fig. 1e), WT and KO FEFs were plated on 1.5-mm
coverslips and immunostained as above. Coverslips were mounted with Vectashield
(Vectorlabs). 3D-SIM imaging was performed on a Zeiss Elyra PS.1 microscope
equipped with a 100x/1.40 oil objective lens. Exciting light was directed
through a movable optical grating to generate a fine-striped interference
pattern on the same plane. Z-stacks of 15 optical sections with a step size of
0.1 μm were acquired to generate images in maximum intensity projection.
The epitope of the ASPM (216-1) antibody43 (link), NDNYGLNQDLESES, is located prior to the TALEN target
site. The following antibodies were used at 1:100 – 1:2000: Centrin
(Millipore 20H5), Par6α (SCBT sc-14405), Par6α (Abcam ab180159),
β-actin (Proteintech 20536-1-AP), ASPM (SCBT sc-98903), ASPM (gift from
J. Bond, 216-1), Ninein (Biolegend Poly6028), aPKCζ (SCBT sc-216).
Johnson M.B., Sun X., Kodani A., Borges-Monroy R., Girskis K.M., Ryu S.C., Wang P.P., Patel K., Gonzalez D.M., Woo Y.M., Yan Z., Liang B., Smith R.S., Chatterjee M., Coman D., Papademetris X., Staib L.H., Hyder F., Mandeville J.B., Grant P.E., Im K., Kwak H., Engelhardt J.F., Walsh C.A, & Bae B.I. (2018). Aspm knockout ferret reveals an evolutionary mechanism governing cerebral cortical size. Nature, 556(7701), 370-375.
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