Fcs express 6

Manufactured by BD

FCS Express 6 is a flow cytometry data analysis software. It provides tools for data visualization, analysis, and reporting.

Automatically generated - may contain errors

Lab products found in correlation

Anti hla a2 pe clone bb7, by BioLegend (2 mentions) Ghost dye 450, by Cytek Biosciences (2 mentions) Lsrfortessa, by BD (2 mentions) Lsm 800 laser scanning microscope, by Zeiss (1 mentions) Hmvec c, by Lonza (1 mentions) Accuri c6 plus flow cytometer, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Rpmi culture medium, by Thermo Fisher Scientific (1 mentions) Lsr 2, by BD (1 mentions)

Spelling variants of this product

FCS Express (8 citations)

7 protocols using fcs express 6

Quantifying Ac-SDKP Uptake in Cardiac Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To examine the uptake of Ac-SDKP by Human Cardiac Microvascular Endothelial Cells (HMVEC-Cs; Cat No. CC-7030, Lonza), we first performed in vitro cell imaging studies using fluorescein isothiocyanate (FITC) conjugated with Ac-SDKP. The FITC-conjugated Ac-SDKP was synthesized using Fmoc solid phase peptide synthesis (Genescript, Piscataway, NJ) and FITC-conjugated scrambled peptide, Ac-KDPS was synthesized using similar Fmoc synthesis protocol (Lifetein, Somerset, NJ). For these studies, peptide uptake was examined in both irradiated and non-irradiated (control) HMVEC-C cells at four hours post peptide treatment. For confocal image analysis, HMVEC-C cells were cultured on sterile cover slips in a 12 well plate. One of the plates was exposed to 9 Gy of radiation using a specialized orthovoltage radiation chamber and a sub group of cells were incubated with FITC-labeled Ac-SDKP or scrambled peptides at 14 μM. Images were captured using a confocal microscope (ZEISS LSM 800 Laser scanning microscope) at 400X magnification. For determining the uptake using FACs, cells were detached, irradiated and incubated with FITC-labeled scrambled peptide or Ac-SDKP (0.9, 1.8, 3.5, 7 & 14 μM). Cell uptake percentage was examined with a BD Accuri C6-Plus flow cytometer and further analyzed by FCS express 6-De Novo Software.

Sharma U.C., Sonkawade S.D., Baird A., Chen M., Xu S., Sexton S., Singh A.K., Groman A., Turowski S.G., Spernyak J.A., Mahajan S.D, & Pokharel S. (2018). Effects of a novel peptide Ac-SDKP in radiation-induced coronary endothelial damage and resting myocardial blood flow. Cardio-oncology, 4, 8.

+ Open protocol

+ Expand

Quantifying HLA-A2 Expression by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess surface HLA-A2 expression, cells were stained on ice for 30 min with anti-HLA-A2 PE (clone BB7.2, BioLegend Cat# 343302) diluted 1:200 in PBS supplemented with 0.2% bovine serum albumin (PBS-BSA) and then washed twice with PBS-BSA. Fluorescence was measured using a BD LSRII and results were analyzed using FCS Express 6. The gating strategies for flow cytometry experiments are shown in Supplementary Fig. 11.

McShan A.C., Devlin C.A., Morozov G.I., Overall S.A., Moschidi D., Akella N., Procko E, & Sgourakis N.G. (2021). TAPBPR promotes antigen loading on MHC-I molecules using a peptide trap. Nature Communications, 12, 3174.

+ Open protocol

+ Expand

Quantifying Cas9-gRNA Efficiency via Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were trypsinized and collected after 48 h post-transfection. Collected cells were resuspended in 500 μL PBS to prepare flow cytometry samples. Samples were analyzed on the LSR II Flow Cytometer (BD Biosciences) and data analysis was performed using FCS Express 6 (Supplementary Fig. 7b). The arithmetic mean of GFP fluorescence was used to compare Cas9-gRNA samples to a reporter only control.

Jain S., Shukla S., Yang C., Zhang M., Fatma Z., Lingamaneni M., Abesteh S., Lane S.T., Xiong X., Wang Y., Schroeder C.M., Selvin P.R, & Zhao H. (2021). TALEN outperforms Cas9 in editing heterochromatin target sites. Nature Communications, 12, 606.

+ Open protocol

+ Expand

Single-Cell Analysis of Mouse Placenta

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ackr3^+/+ and Ackr3^−/− placentas (n=4/per run) from pregnant mice at e12.5 were genotyped by determining GFP signal in each embryo and then minced and enzymatically digested (StemPro Accutase, A1110501) on ice. Each genotype was later validated via PCR. Following tissue dissociation, the samples were incubated at 37°C for 30 min and then passed through a sterile cell strainer to obtain a single-cell suspension. Leukocytes and stromal cells were obtained by density gradient centrifugation (1250 × g, 10 min at 4°C). The cell pellet was then re-suspended with 1 ml RPMI culture medium (Gibco ™, 21875034), and then 500 μl of neat FBS was slowly overlaid above the cell suspension. To pellet viable cells, the suspension was centrifuged for 10 min at 1100 × g at room temperature. The cell pellet was incubated for 30 min. at 4°C with a viability dye (Live/Dead fixable yellow, L34967). The cells were then blocked for non-antigen-specific binding of immunoglobins (CD16/CD32, BD Pharmingen™, 553141) for 10 min., and then antibodies were added (suppl. table 1). Following a 30 min. incubation of antibodies, cells were fixed and then acquired immediately using a Becton Dickinson LSR II and FACSDiva 8.0.1 acquisition software. Results were analyzed using FCS Express 6 (BD Biosciences).

Quinn K.E., Matson B.C, & Caron K.M. (2020). Deletion of Atypical Chemokine Receptor 3 (ACKR3) increases immune cells at the fetal-maternal interface. Placenta, 95, 18-25.

+ Open protocol

+ Expand

HLA-A2 Surface Expression Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess surface HLA-A2 expression, cells were stained on ice for 30 minutes with anti-HLA-A2 PE (clone BB7.2, BioLegend) diluted 1:200 in PBS supplemented with 0.2% bovine serum albumin (PBS-BSA) and then washed twice with PBS-BSA. Fluorescence was measured using a BD LSRII and results were analyzed using FCS Express 6.

McShan A.C., Devlin C.A., Morozov G.I., Overall S.A., Moschidi D., Akella N.M., Procko E., & Sgourakis N.G. (2020). TAPBPR Promotes Antigen Loading on MHC-I Molecules Using a Peptide Trap.

+ Open protocol

+ Expand

Cell Surface and Intracellular Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols

For surface marker staining, dissociated cells were incubated with conjugated antibodies for 1 hour at 4°C and were stained with DAPI (1 μg/mL) to exclude dead cells. For intracellular staining, cells were stained with Ghost Dye 450 (TONBO biosciences, 13-0868) prior to 4% PFA fixation to stain dead cells. Fixed cells were permeabilized in PBS with 5% donkey serum and 0.3% Triton X-100 for 30 mins at room temperature. Cells were incubated with primary antibodies in 1x PBS-/- with 5% donkey serum and 0.1% Triton X100 overnight at 4˚C. The following day, cells were washed twice in 1x PBS and unconjugated antibodies were further incubated with secondary antibodies (Alexa Fluor conjugates) for 2 hours. Antibody sources and concentrations are indicated in Supplementary Table 1. Cells were analysed using an LSR Fortessa (BD Bioscience) or FACS sorted by SH800 (SONY SH800 Software). All data were analysed with FCS Express 6 software (BD Biosciences). Antibody information is listed on Supplementary Table 1.

Minter R.M., Ferry M.A., Rectenwald J.E., Bahjat F.R., Oberholzer A., Oberholzer C., La Face D., Tsai V., Ahmed C.M., Hutchins B., Copeland EM I.I.I., Ginsberg H.S, & Moldawer L.L. (2000). Extended lung expression and increased tissue localization of viral IL-10 with adenoviral gene therapy. Proceedings of the National Academy of Sciences of the United States of America, 98(1), 277-282.

+ Open protocol

+ Expand

Comprehensive Cell Staining and Sorting

Check if the same lab product or an alternative is used in the 5 most similar protocols

For surface marker staining, dissociated cells were incubated with conjugated antibodies for 1 h at 4 °C and were stained with DAPI (1 μg ml⁻¹) to exclude dead cells. For intracellular staining, cells were stained with Ghost Dye 450 (TONBO Biosciences, 13-0868) before 4% paraformaldehyde fixation to stain dead cells. Fixed cells were permeabilized in PBS with 5% donkey serum and 0.3% Triton X-100 for 30 min at room temperature. Cells were incubated with primary antibodies in 1× PBS−/− with 5% donkey serum and 0.1% Triton X-100 overnight at 4 °C. The following day, cells were washed twice in 1× PBS and unconjugated antibodies were further incubated with secondary antibodies (Alexa Fluor conjugates) for 2 h. Antibody sources and concentrations are indicated in Supplementary Table 8. Cells were analysed using an LSR Fortessa (BD Bioscience) or FACS sorted by SH800 (SONY SH800 Software). All data were analysed with FCS Express 6 software (BD Biosciences). Antibody information is listed in Supplementary Table 8.

Wong Y.F., Kumar Y., Proks M., Herrera J.A., Rothová M.M., Monteiro R.S., Pozzi S., Jennings R.E., Hanley N.A., Bickmore W.A, & Brickman J.M. (2023). Expansion of ventral foregut is linked to changes in the enhancer landscape for organ-specific differentiation. Nature Cell Biology, 25(3), 481-492.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!