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Bv421 tcrβ clone h57 597

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BV421-TCRβ (clone H57–597) is a fluorochrome-conjugated antibody that binds to the TCRβ chain, a component of the T cell receptor complex. It can be used for the flow cytometric detection and characterization of T cells.

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Lab products found in correlation

Percp cy5.5 cd4 clonerm4 5, by BioLegend (3 mentions) Bv 711 cd8 clone 53 6.7, by BioLegend (3 mentions) Pe cd25 clone pc61, by BioLegend (2 mentions) Aryacell sorter, by BD (2 mentions) Monensin solution, by BioLegend (1 mentions) Cell activation cocktail, by BioLegend (1 mentions) Intracellular fixation permeabilization buffer set, by Thermo Fisher Scientific (1 mentions) Live dead fixable yellow dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Facs aurora, by Cytek (1 mentions)

Spelling variants of this product

H57-597 (19 citations) TCRβ (H57-597, (17 citations) TCRβ (clone H57-597), (6 citations)

3 protocols using bv421 tcrβ clone h57 597

1

Isolation and RNA Extraction of Foxp3+ T Cells

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DEREG (eGFP.Foxp3) reporter mice were used for cell sorting. Single
cell suspensions were obtained from BM and stained with CD16/32,BV
421-TCRβ (clone H57–597), PerCP/Cy5.5-CD4 (clone
RM4–5), PE-CD25 (clone PC61) and BV 711-CD8-(clone 53–6.7)
(Biolegend, San Diego, CA). 1 X106 cells were sorted on a BD Arya
cell sorter. The sorted cells were collected in complete RPMI-1640 media.
Purified cells were then collected by centrifugation and dissolved in Trizol
reagent for RNA isolation.
Tyagi A.M., Yu M., Darby T.M., Vaccaro C., Li J.Y., Owens J.A., Hsu E., Adams J., Weitzmann M.N., Jones R.M, & Pacifici R. (2018). The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. Immunity, 49(6), 1116-1131.e7.
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2

Isolation and RNA Extraction of Foxp3+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
DEREG (eGFP.Foxp3) reporter mice were used for cell sorting. Single
cell suspensions were obtained from BM and stained with CD16/32,BV
421-TCRβ (clone H57–597), PerCP/Cy5.5-CD4 (clone
RM4–5), PE-CD25 (clone PC61) and BV 711-CD8-(clone 53–6.7)
(Biolegend, San Diego, CA). 1 X106 cells were sorted on a BD Arya
cell sorter. The sorted cells were collected in complete RPMI-1640 media.
Purified cells were then collected by centrifugation and dissolved in Trizol
reagent for RNA isolation.
Tyagi A.M., Yu M., Darby T.M., Vaccaro C., Li J.Y., Owens J.A., Hsu E., Adams J., Weitzmann M.N., Jones R.M, & Pacifici R. (2018). The Microbial Metabolite Butyrate Stimulates Bone Formation via T Regulatory Cell-Mediated Regulation of WNT10B Expression. Immunity, 49(6), 1116-1131.e7.
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3

Comprehensive Immune Cell Profiling

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Flow cytometry was performed on a FACS Cytek Aurora (Cytek Bioscience), and data were analyzed using FlowJo software (Tree Star Inc.). For cell-surface staining, cells were stained with anti-mouse purified CD16/32 (Fc blocking Ab, clone 93, catalog 101302), BV 510-CD45 (clone 30-F11, catalog 103138), BV 421-TCR-β (clone H57-597, catalog 109230), Alexa488-CD3 (clone 17A2, catalog 100210), PerCP/Cy5.5-CD4 (clone RM4-5, catalog 100540), BV 711-CD8 (clone 53-6.7, catalog 100748) (all from BioLegend), The live cells were discriminated by the LIVE/DEAD Fixable Yellow Dead Cell Stain Kit (Thermo Fisher Scientific). For intracellular staining, cells were incubated with cell activation cocktail (BioLegend) in the presence of Monensin Solution at 37°C for 12 hours. Anti-mouse APC-FOXP3 (clone FJK-16s, catalog 17-5773-82) (Thermo Fisher Scientific) was added after cell fixation and permeabilization with Intracellular Fixation & Permeabilization Buffer Set (Thermo Fisher Scientific).
Pal S., Morgan X., Dar H.Y., Gacasan C.A., Patil S., Stoica A., Hu Y.J., Weitzmann M.N., Jones R.M, & Pacifici R. (2024). Gender-affirming hormone therapy preserves skeletal maturation in young mice via the gut microbiome. The Journal of Clinical Investigation, 134(10), e175410.
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