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Hank s balanced salt solution hbss powder form

Manufactured by Merck Group
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Sourced in United States, Germany

Hank's Balanced Salt Solution (HBSS) is a powdered formulation that provides a balanced salt solution used for a variety of cell culture and biological applications. It maintains the osmotic and ionic balance necessary for the survival and normal functioning of cells in vitro.

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Lab products found in correlation

Magnesium chloride, by Merck Group (2 mentions) Bca protein assay kit, by Thermo Fisher Scientific (2 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Milli q water, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Daidzin, by Merck Group (1 mentions) Acetonitrile, by Avantor (1 mentions) Methanol, by Avantor (1 mentions) D saccharic 1, by Merck Group (1 mentions) 4 lactone monohydrate, by Merck Group (1 mentions) Lc ms grade formic acid, by Avantor (1 mentions) Transwell cell culture insert, by Corning (1 mentions) Ko143, by Merck Group (1 mentions) Mk571, by Merck Group (1 mentions) Sn 38, by LGC (1 mentions) Sn 38g, by LGC (1 mentions) Uridine 5 diphosphate β, by Merck Group (1 mentions) Hepes, by Merck Group (1 mentions) Nahco3, by Merck Group (1 mentions)

Close spelling variants of this product

Hanks’ Balanced Salts (HBSS) Hank’s balanced salts Hank’s balanced solution Hanks′ Balanced Salt Balanced salt solution Hank’s solution HBSS solution S powder

4 protocols using hank s balanced salt solution hbss powder form

1

Rosmarinic Acid and SalB Nanoparticles

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Rosmarinic acid (99.8%) and SalB (99.5%) were purchased from the National Institute for the Control of Pharmaceutical and Biological Products (NICPBP, Beijing, China). Water-soluble CS with various MWs (3, 30, 50, and 100 kDa) were supplied by KITTOLIFE (Seoul, Korea). Cloned Caco-2 TC7 cells were a generous gift from Dr. Ming Hu of INSERM U178 (Houston, TX). Sodium TPP was purchased from Merck (Darmstadt, Germany). 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) and Hank's balanced salt solution (HBSS; powder form) were purchased from Sigma-Aldrich (St. Louis, MO). Milli-Q water (Millipore, Bedford, MA) was used throughout the study. All the reagents were of analytical grade, except acetonitrile and methanol, which were chromatographic grade (Merck Company Inc., UN).
Jin X., Zhang S.B., Li S.M., Liang K, & Jia Z.Y. (2016). Influence of Chitosan Nanoparticles as the Absorption Enhancers on Salvianolic acid B In vitro and In vivo Evaluation. Pharmacognosy Magazine, 12(45), 57-63.
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2

Optimized Irinotecan Formulation Preparation

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irinotecan HCl Trihydrate injection (20 mg/mL) were purchased from Teva Parenteral Medicine, Inc. (Irvine, NY, USA) and the powder of irinotecan were purchased from Dalian Meilun Biology Technology Co., Ltd. (Dalian, China), respectively. The self-made injectable irinotecan formulation was prepared as follows: the irinotecan hydrochloride was firstly dissolved in 1,3 Propanedio by sonication 10 min, then diluted in a sorbitol/lactic acid buffer (D-sorbitol 45 mg/ml and D-lactic acid 0.9 mg/ml, pH=3.4), the control group was given 0.2 mL sorbitol/lactic acid buffer.
The reference standard irinotecan, SN-38, SN-38G, NPC, and APC were purchased from Toronto Research Chemicals Inc. (Toronto Canada). Uridine-5’- diphosphate-β, D-glucuronic acid ester, D-saccharic-1, 4-lactone monohydrate, magnesium chloride, daidzin, and astragaloside were purchased from Sigma–Aldrich Co. (St. Louis, MO, USA). Acetonitrile, methanol, and formic acid (LC-MS grade) were purchased from VWR. Glucose, Hank’s balanced salt solution (HBSS, powder form), HEPES, Ko143, MK-571, Zosuquidar (LY335979), NaHCO3, and fetal bovine serum were purchased from Sigma-Aldrich Co., (St. Louis, MO, USA). Transwell™ cell culture insert (polycarbonate membrane and polystyrene plates with a diameter of 24 mm and a pore size of 3 μm) was obtained from Corning Incorporated (Corning, NY, USA).
Sun R., Zhu L., Li L., Song W., Gong X., Qi X., Wang Y., Ghose R., Gao S., Hu M, & Liu Z. (2020). Irinotecan-mediated diarrhea is mainly correlated with intestinal exposure to SN-38: critical role of gut Ugt.. Toxicology and applied pharmacology, 398, 115032.
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3

Caco-2 Permeability Assay Protocol

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Transwell permeable polycarbonate inserts (0.4 μm) and 12-well cell culture plates were obtained from Corning. (Cambridge, MA, USA). The Caco-2 cell line was generously provided by the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Gibco Laboratories (Life Technologies Inc., Grand Island, NY, USA). Hanks’ balanced salt solution (HBSS, powder form) was obtained from Sigma Chemical Co. (Deisenhofen, Germany). Fetal bovine serum was purchased from HyClone (Logan, UT, USA). The 100× nonessential amino acids, 100× penicillin and streptomycin, 0.25% trypsin with ethylene-diaminetetraacetic acid (EDTA), 1 M 4-(2-hydroxyethyl)-1-piperazi- neethanesulfonic acid (HEPES), and bovine serum albumin were purchased from Invitrogen Corp. (Carlsbad, CA, USA). Verapamil, MK571, indomethacin, benzbromarone, apigenin, sodium vanadate and cimetidine used in this study were obtained from Aladdin Industrial Inc. (Shanghai, China). Morroniside (purity > 98.0%) and loganin (internal standard (IS), purity > 98.0%) were obtained from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). All other reagents (typically of analytical grade or better) were used as received.
Xu R., Zhu H., Hu L., Yu B., Zhan X., Yuan Y, & Zhou P. (2020). Characterization of the intestinal absorption of morroniside from Cornus officinalis Sieb. et Zucc via a Caco-2 cell monolayer model. PLoS ONE, 15(5), e0227844.
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4

Phytochemical Glucuronidation Assay

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Genistein, biochanin A, apigenin and chrysin were purchased from Indofine Chemicals (Hillsborough, NJ). Hanks’ balanced salt solution (HBSS, powder form), uridine-diphosphoglucuronic acid (UDP), alamethicin, D-saccharic-1,4-lactone monohydrate, and magnesium chloride were purchased from Sigma-Aldrich (St. Louis, MO). BCA protein assay kit was purchased from Thermo Scientific (Rockford, IL). Non-soy rat chow (AIN76A) was purchased from Harlan Laboratory (Madison, WI). All other materials (typically analytical grade or better) were used without further purification.
Zeng M., Sun R., Basu S., Ma Y., Ge S., Yin T., Gao S., Zhang J, & Hu M. (2016). Disposition of Flavonoids via Recycling: Direct Biliary Excretion of Enterically or Extrahepatically Derived Flavonoid Glucuronides. Molecular nutrition & food research, 60(5), 1006-1019.
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