N acetyllactosamine

Manufactured by Biosynth

Sourced in United Kingdom

N-acetyllactosamine is a chemical compound that serves as a core glycan structure. It is commonly used in biochemical research and laboratory applications.

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Lab products found in correlation

Lacto n biose, by Biosynth (2 mentions) 3 sialyllactose, by Biosynth (1 mentions) 6 sialyllactose, by Biosynth (1 mentions) Recombinant human vascular endothelial growth factor, by R&D Systems (1 mentions) Lactose, by Biosynth (1 mentions) 3 sialyllactosamine, by Biosynth (1 mentions) Igepal ca 630, by Merck Group (1 mentions) 3 sialyllactose sodium salt, by Biosynth (1 mentions) 6 sialyllactose sodiumsalt, by Biosynth (1 mentions) Cmp neu5ac disodium salt, by Biosynth (1 mentions) Benzyl 2 acetamido 2 deoxy 3 o β d galactopyranosyl α d galactopyranoside gal β 1 3 galnac α o bn, by Biosynth (1 mentions) Nucleoside monophosphate kinase nmpk, by Roche (1 mentions) Lactate dehydrogenase pyruvate kinase solution pk ldh, by Merck Group (1 mentions) Valinomycin, by Merck Group (1 mentions) Lacto n tetraose, by Biosynth (1 mentions) Lacto n neotetraose, by Biosynth (1 mentions) Polymyxin b, by Merck Group (1 mentions) Cyclosporin a, by Merck Group (1 mentions) Lc ms grade solvents, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

3′-sialyl-N-acetyllactosamine (2 citations)

3 protocols using n acetyllactosamine

Oligosaccharides Modulate VEGFR-2 Signaling

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The oligosaccharides of human milk, such as lactose, lacto N-biose, N-acetyllactosamine, 3′-sialyllactosamine, 6′-sialyllactosamine, 3′-sialyllactose (3SL), and 6′-sialyllactose (6SL), were purchased from Carbosynth Ltd. (Berkshire, UK). Recombinant human vascular endothelial growth factor (VEGF) was supplied by R&D systems Inc. (Minneapolis, MN, USA). Antibodies against the phosphorylated form or total form of VEGF receptor-2 (VEGFR-2), extracellular signal-regulated kinase (ERK), Akt, and p38 were purchased from Cell Signaling Technology (Danvers, MA, USA). The antibody against von Willebrand Factor (vWF) was purchased from Dako (CA, USA). Antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and hoseradish peroxide (HRP)-conjugated secondary antibodies were supplied by Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Chung T.W., Kim E.Y., Kim S.J., Choi H.J., Jang S.B., Kim K.J., Ha S.H., Abekura F., Kwak C.H., Kim C.H, & Ha K.T. (2017). Sialyllactose suppresses angiogenesis by inhibiting VEGFR-2 activation, and tumor progression. Oncotarget, 8(35), 58152-58162.

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Enzymatic Synthesis of Sialylated Glycans

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CMP-Neu5Ac disodium salt, benzyl 2-acetamido-2-deoxy-3-O-(β-d-galactopyranosyl)-α-d-galactopyranoside (Gal-β-1,3-GalNAc-α-O-Bn), N-acetyllactosamine, 6'-α-sialyl-N-acetyllactosamine sodium salt, 3'-α-sialyl-N-acetyllactosamine sodium salt, 6'-sialyllactose sodium salt and 3'-sialyllactose sodium salt were purchased from Carbosynth (Berkshire, UK). Nucleoside Monophosphate Kinase (NMPK) was purchased from Roche (Mannheim, Germany). Lactate dehydrogenase/pyruvate kinase solution (PK/LDH) and IGEPAL-CA-630 were purchased from SIGMA (Steinheim, Germany). Phosphoenolpyruvic acid monopotassium salt (PEP) and NADH were acquired from Alfa Aeasar (Karlsruhe, Germany) and AppliChem (Darmstadt, Germany) respectively. TLC silica gel 60 F₂₄₀ plates were obtained from Merck (Darmstadt, Germany) and electrophoresis reagents from Merck and BDH-Prolabo (Darmstadt, Germany).

Ortiz-Soto M.E, & Seibel J. (2016). Expression of Functional Human Sialyltransferases ST3Gal1 and ST6Gal1 in Escherichia coli. PLoS ONE, 11(5), e0155410.

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HMO Standards Characterization

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Human milk oligosaccharide standards were purchased from Carbosynth (Berkshire, UK): lacto-N-biose (LNB), N-acetyl lactosamine (LacNAc), lacto-N-tetraose (LNT), lacto-N-neotetraose (LNnT), para-Lacto-N-hexaose (pLNH), and para-Lacto-N-neohexaose (pLNnH). 3-amino 3-deoxy α-cyclodextrin (α-CD) and 3-amino 3-deoxy β-cyclodextrin (β-CD) were purchased from Tokyo Chemical Industry (Tokyo, Japan). Cucurbit[5]uril (CB5), cucurbit[7]uril (CB7), cyclosporin A, valinomycin, polymyxin B, and ammonium ionophore I were purchased from Sigma-Aldrich (Milwaukee, WI USA). 12-Crown-4, 15-Crown-5, and 18-Crown-6 were purchased from Alfa Aesar (Ward Hill, MA USA). LC-MS grade solvents were obtained from Fisher Scientific (Pittsburgh, PA USA). Stock solutions for all HMOs, cyclodextrins, cucurbiturils, crown ethers, and polymyxin B were dissolved in 100% H₂O, while stock solutions for cyclosporin A, valinomycin, and ammonium ionophore I were dissolved in 100% methanol. Each host-guest solution mixture was prepared to 5 μM in 49.75/49.75/0.5 (v/v) water/methanol/acetic acid for each reagent used (i.e., both the host and guest were at 5 μM). Each HMO pair was also run as a mixture (see the solid black traces in Figures S1–S3 of the Supporting Information).

Habibi S.C, & Nagy G. (2022). Assessing the Use of Host-Guest Chemistry in Conjunction with Cyclic Ion Mobility Separations for the Linkage-Specific Characterization of Human Milk Oligosaccharides. International journal of mass spectrometry, 483, 116977.

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