Ab131165

Manufactured by Abcam

Ab131165 is a lab equipment product offered by Abcam. It is a primary antibody used for detection and quantification of target proteins in various applications, such as Western blotting, immunohistochemistry, and ELISA.

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Lab products found in correlation

Pageblue protein staining solution, by Thermo Fisher Scientific (2 mentions) F7425, by Merck Group (1 mentions) H6908, by Merck Group (1 mentions) A105239, by Merck Group (1 mentions) Act 2d7, by Euromedex (1 mentions) A1978, by Merck Group (1 mentions)

2 protocols using ab131165

Protein Detection and Analysis

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SDS–PAGE and Western blot analysis were performed as previously described 54 using the following antibodies: rabbit anti‐GFP (1:5,000; GFP‐2A3, Merck), rabbit anti‐FLAG (1:1,000; F7425, Sigma), rabbit anti‐HA (1:1,000; H6908, Sigma), rabbit anti MOSPD2 (1:1,000; A105239, Sigma), mouse anti‐MOSPD2 (1:2; 1MOS‐4E10), goat anti‐VAP‐A (1:1,000; K‐15, Santa Cruz Biotechnology, sc‐48698), mouse anti‐VAP‐A (1:1,000; 4C12, Santa Cruz Biotechnology, sc‐293278), rabbit anti‐VAP‐B (kind gift from Dr. L. Dupuis 61), rabbit anti‐STARD3 (1:1,000; pAbMLN64‐Ct‐605; 58), rabbit anti‐STARD3NL (1:1,000; pAbMENTHO‐Ct‐1545; 53), rabbit anti‐ORP1 (1:1,000; abcam; ab131165), and mouse anti‐actin (1:5,000; ACT‐2D7, Euromedex).
Coomassie blue staining was performed with PageBlue Protein Staining Solution (Thermo Fisher Scientific).

Di Mattia T., Wilhelm L.P., Ikhlef S., Wendling C., Spehner D., Nominé Y., Giordano F., Mathelin C., Drin G., Tomasetto C, & Alpy F. (2018). Identification of MOSPD2, a novel scaffold for endoplasmic reticulum membrane contact sites. EMBO Reports, 19(7), e45453.

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SDS-PAGE and Western Blot Analysis

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SDS–PAGE and Western blot analysis were performed as previously described (Alpy et al, 2005 (link)) using the following antibodies: rabbit anti-STARD3NL (1:1,000; pAbMENTHO-Ct-1545 [Alpy et al, 2001 (link)]), rabbit anti-ORP1 (1:1,000; ab131165; Abcam), and mouse anti-actin (1:5,000; A1978; Merck). Coomassie blue staining was performed with PageBlue Protein Staining Solution (Thermo Fisher Scientific).

Mishra S., Manohar V., Chandel S., Manoj T., Bhattacharya S., Hegde N., Nath V.R., Krishnan H., Wendling C., Di Mattia T., Martinet A., Chimata P., Alpy F, & Raghu P. (2024). A genetic screen to uncover mechanisms underlying lipid transfer protein function at membrane contact sites. Life Science Alliance, 7(6), e202302525.

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