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Methylene blue

Manufactured by Daejung Chemicals

Methylene blue is a synthetic dye commonly used as a laboratory reagent. It is a dark blue or purple crystalline solid that is soluble in water and alcohol. Methylene blue has a variety of applications in chemical analysis and biological staining, but its core function is as a redox indicator, changing color based on its oxidation state.

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3 protocols using methylene blue

1

Synthesis of Graphite-Ilmenite Composite

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Ilmenite sand with a particle size of 100–200 µm was supplied by Lanka Mineral Sand (Pvt) Ltd. Graphite was obtained from Bogala, Sri Lanka. Hydrochloric acid (37%, HCl) and 2-propanol (99.95%, C3H7OH) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Ammonium hydroxide (29%, NH4OH) was purchased from Merk & Co., Inc., Rahway, NJ, USA. Ferrous sulfate heptahydrate (99.5%, FeSO4.7H2O) and L-ascorbic acid (C6H8O6) were sourced from Sisco Research Laboratories in India. Methylene Blue was bought from Daejung Chemical & Metal Co., Ltd. (Shiheung, South Korea). All reagents used were analytical grade and solutions were prepared using ultra-pure water.
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2

LAMP Assay for Pathogen Detection

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Reaction mixtures including Tris–HCl, KCl, MgSO4, (NH4)SO4, Tween20, Betaline, dNTPs and BST DNA polymerase for LAMP and fluorescent detection reagent were purchased from Eiken Co. Ltd. (Tokyo, Japan). Methylene blue was provided by Daejung Chemicals & Metals Co. Ltd. (Siheung, Korea). LAMP primers in aqueous form were purchased from Sigma Aldrich Pte Ltd. (Singapore). Deionized water was purchased from RCI Labscan Ltd. (Pathumwan, Thailand). The universal set of LAMP primers (Sigma Aldrich, Singapore) which was able to detect all available genotypes (A to F),11 (link) was used in the present study (Table 1).
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3

Synthesis of Metal-Dye Composites

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The metal precursors (Ni(NO3)2·6H2O) (< 99.95%), (PdCl2) (< 99.99%), (Na2CO3) (< 99.99%) and (KOH) (extra pure) all chemicals were obtained from Dae-Jung chemicals, Korea. The dyes such as Methylene blue, Rhodamine B, and Methyl orange were purchased from Dae-Jung chemicals, Korea. The Aromatic nitrophenols (NP, DNP, TNP) and NaBH4 (< 99.99%) were purchased from Sigma Aldrich, Korea.
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