RIP was performed using a Magna RIPTM RNA kit (Millipore, USA). Briefly, cultured chondrocytes were suspended in RIP lysis buffer (Solarbio) and incubated in RIP buffer containing human anti-Ago2 antibody beads (Millipore) overnight (Input and normal IgG served as controls). Next, RNAs were extracted using TRIzol reagent to follow the relative enrichment of MEG3/FOXO1 and miR-361-5p.
Human anti ago2 antibody beads
Manufactured by Merck Group
The Human anti-Ago2 antibody beads are a lab equipment product used for isolating and studying the Argonaute 2 (Ago2) protein, which is a key component of the RNA-induced silencing complex (RISC). These beads are coated with antibodies specific to the Ago2 protein, allowing for the immunoprecipitation and purification of Ago2 and associated RNA molecules from cell lysates or other biological samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using human anti ago2 antibody beads
1
Analyzing Ago2-Mediated RNA Interactions
2
RIP Analysis of LINC00485/Twist1 and miR-361-5p
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Using a Magna RIPTM RNA kit (Millipore, USA), RIP analysis was carried out. Cultured chondrocytes were suspended in RIP lysis buffer and were further incubated overnight in RIP buffer that contained human anti-Ago2 antibody beads (Millipore) (Input and normal IgG served as controls). Afterward, RNA extraction was achieved using the TRIzol reagent and the relative enrichment of LINC00485/Twist1 and miR-361-5p was tested via the RT-PCR analysis.
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