Phase contrast microscopy was performed using stationary phase cultures. Bacteria were immobilized on LB pads containing 1% agarose. Image acquisition was performed using a Zeiss Axio Imager.Z2 microscope equipped with a Plan-Apochromat 63X phase contrast objective lens and an ORCA-Flash 4.0 LT digital CMOS camera (Hamamatsu), using the Zeiss Zen Blue software. For image processing and analysis the MicrobeJ plugin for Fiji was used (Ducret et al., 2016 (link)). Cell length and width from 570 to 700 cells per strain (three fields of 190–250 cells each) were measured and statistical significance was calculated using a t-test (unpaired).
Plan apochromat 63x phase
Manufactured by Hamamatsu Photonics
Sourced in Germany
The Plan-Apochromat 63X phase is a high-magnification objective lens designed for use in optical microscopy. It features apochromatic correction for improved color fidelity and a plan-corrected flat field for uniform image quality across the field of view. The lens is optimized for phase contrast imaging, providing enhanced contrast for visualizing transparent or low-contrast samples.
Automatically generated - may contain errors
3 protocols using plan apochromat 63x phase
1
Bacterial Cell Morphometry by Phase Contrast
2
Phase Contrast Microscopy of Stationary Bacteria
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Stationary phase bacteria were placed on 1% agarose LB pads. Phase contrast microscopy was done using a Zeiss Axio Imager.Z2 microscope (Zeiss, Oberkochen, Germany) equipped with a Plan-Apochromat 63X phase contrast objective lens and an ORCA-Flash 4.0 LT digital CMOS camera (Hamamatsu Photonics, Shizuoka, Japan), using the Zeiss Zen Blue software.
3
Phase Contrast Microscopy of Stationary Bacteria
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Stationary phase bacteria were placed on 1% agarose LB pads. Phase contrast microscopy was done using a Zeiss Axio Imager.Z2 microscope (Zeiss, Oberkochen, Germany) equipped with a Plan-Apochromat 63X phase contrast objective lens and an ORCA-Flash 4.0 LT digital CMOS camera (Hamamatsu Photonics, Shizuoka, Japan), using the Zeiss Zen Blue software.
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