Pe conjugated goat anti mouse secondary antibodies

Cells were harvested using the non‐enzymatic dissociation buffer Versene (Life Technologies) and collected by centrifugation. Cells were exposed to an anti‐CD324 antibody (E‐cadherin, clone 180224, R & D Systems, Minneapolis, MN). For intracellular staining, cells were exposed to Flow Cytometry Fixation Buffer (R & D Systems) for 20 min at 4 °C in the dark, followed by the addition of Flow Cytometry Permeabilization buffer for 5 min at room temperature (R & D Systems). Cells were exposed to anti‐Vimentin (clone RV202) and anti‐Fibronectin antibodies (clone 10, BD Biosciences, San Jose, CA, used also for western blot) all at optimized antibody dilutions. Primary antibodies were recognized by PE‐conjugated goat anti‐mouse secondary antibodies (Life Technologies) on an LSR II Flow Cytometer (BD Biosciences).