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Hp β cd

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HP-β-CD is a chemically modified form of beta-cyclodextrin, a cyclic oligosaccharide. It is used as a solubilizing agent and complexing agent in pharmaceutical and analytical applications.

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Lab products found in correlation

Orthophosphoric acid, by Merck Group (1 mentions) Gallic acid, by Extrasynthese (1 mentions) Isogentisin, by Phytolab (1 mentions) Folin ciocalteu s phenol reagent, by Merck Group (1 mentions) Milli q water purification system, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Sodium carbonate, by Merck Group (1 mentions) Hplc grade acetonitrile, by Merck Group (1 mentions) Gentiopicroside, by ChromaDex (1 mentions)

4 protocols using hp β cd

1

Purification and Characterization of MTX

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MTX (≥99%) was supplied by Huzhou Zhanwang Pharm. Co., Ltd. (Huzhou, China). The β-CD, HP-β-CD, M-β-CD, and DM-β-CD were obtained from Acros Organics (Fair Lawn, NJ, USA). All other chemicals and solvents were of reagent grade and were used as received.
Giri B.R., Yang H.S., Song I.S., Choi H.G., Cho J.H, & Kim D.W. (2022). Alternative Methotrexate Oral Formulation: Enhanced Aqueous Solubility, Bioavailability, Photostability, and Permeability. Pharmaceutics, 14(10), 2073.
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2

hGH Extraction and ELISA Analysis

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The human growth hormone (hGH) was obtained from Novo Nordisk A/S. hGH ELIZA kit was purchased from PADTAN ELM (Iran): HP-β-CD from Acros organics.
Urea and other chemicals from Aldrich chemical company, Inc. Rats used in animal studies were obtained from the Pasteur Research Institute (Iran).
Shams L., Khodabandeh Shahraky M, & Mirtaleb M.S. (2021). Transdermal Co-Delivery of Urea and Recombinant Human Growth Hormone. Iranian Journal of Biotechnology, 19(4), e2891.
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3

Synthesis and Characterization of CXCR1/2 Inhibitor

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SCH-479833 [46 (link)] was synthesized at Schering-Plough (Kenilworth, NJ) and formulated in hydroxypropyl-beta-cyclodextrin (HPβCD, Acros Organics, Morris Plains, NJ). The inhibition constant (Ki) of CXCR1 (17 nM) and CXCR2 (0.17 nM) for SCH-479833 were calculated from the IC50 value using the Cheng-Prusoff equation [47 (link)].
Ames S.K., Frankema N, & Kenney L.J. (1999). C-terminal DNA binding stimulates N-terminal phosphorylation of the outer membrane protein regulator OmpR from Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America, 96(21), 11792-11797.
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4

Phytochemical Analysis of Medicinal Plants

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Ultrapure water was prepared using a Milli-Q water purification system (Millipore, France). HPβCD (97%) was obtained from Acros Organics. Folin–Ciocalteu′s phenol reagent, sodium carbonate, orthophosphoric acid, and methanol were purchased from Sigma–Aldrich Chemie GmbH (Munich, Germany), while HPLC-grade acetonitrile was purchased from Merck (Darmstadt, Germany). Gallic acid (Extrasynthese, Genay, France), gentiopicroside (ChromaDex, Los Angeles, CA, USA), and isogentisin (Phytolab, Vestenbergsgreuth, Germany) were used as standards.
Jovanović M.S., Krgović N., Šavikin K, & Živković J. (2022). Ultrasound-Assisted Water Extraction of Gentiopicroside, Isogentisin, and Polyphenols from Willow Gentian “Dust” Supported by Hydroxypropyl-β-Cyclodextrin as Cage Molecules. Molecules, 27(21), 7606.
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