One half of brain (cut longitudinally) was frozen for western blot (WB) as described previously. [28] (link) Portions of brainstem from each sheep were sampled for WB examination. Briefly, monoclonal P4 (R-Biopharm, Inc., Marshall, MI) as primary antibody and biotinylated anti-mouse (dilution 1∶10,000; Biotinylated anti-mouse IgG, Amersham Biosciences, USA) as secondary antibody, followed by streptavidin horseradish-peroxidase conjugate (dilution 1∶10.000; streptavidin horseradish-peroxidase conjugate, Amersham Biosciences, USA) were utilized. The immunoblot was developed with a chemiluminescence solution (ECL Plus, Amersham Biosciences, USA) before being scanned by an imaging system.
Streptavidin horseradish peroxidase conjugate
Manufactured by Cytiva
Sourced in United States
Streptavidin horseradish-peroxidase conjugate is a protein complex composed of streptavidin, which binds to biotin, and horseradish peroxidase, an enzyme. This conjugate is used as a detection reagent in various biotechnology applications, such as immunoassays and Western blotting, where it provides a signal that can be measured to quantify the presence of target molecules.
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Lab products found in correlation
2 protocols using streptavidin horseradish peroxidase conjugate
1
Western Blot Analysis of Brain Samples
2
PrP Sc Molecular Characterization
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To evaluate the molecular characteristics of PrP Sc , Western blot detection was performed on the frozen sections of brainstem at the level of the obex from representative samples of each genotype and inoculation group. The assay was performed as previously described 16 using monoclonal antibody P4 (0.1 mg/ml; R-Biopharm, Marshall, MI), a biotinylated anti-mouse secondary antibody (0.1 mg/ml; biotinylated anti-mouse IgG, Amersham Biosciences, Waltham, MA), and streptavidin horseradish peroxidase conjugate (dilution 1:10 000; streptavidin horseradish-peroxidase conjugate, Amersham Biosciences). 16 A chemiluminescent detection system was used to acquire images.
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