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Evos fl auto 2 imaging microscope

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The EVOS FL Auto 2 Imaging Microscope is a fully automated, inverted fluorescence microscope designed for live-cell imaging. It features advanced optics, seamless digital acquisition, and intuitive software for capturing high-quality images and time-lapse videos.

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2 protocols using evos fl auto 2 imaging microscope

1

Evaluating SBH Cytoprotection Against H2O2

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Seeded cells, (1 × 105 cells/well) in 24 well plates after 24 h were treated with different concentrations of SBH for 1 h. The wells apart from the control were stimulated with H2O2 (1 mM) and incubated for another 24 h. Hoechst 33342 (10 μg/mL) was then added and incubated for 10 min. Afterward, the culture media was removed and new media containing PI was added to the wells and visualized using the EVOS FL Auto 2 Imaging microscope (ThermoFisher Scientific, Waltham, CA, USA) [15 (link)].
Fernando I.P., Kirindage K.G., Jayasinghe A.M., Han E.J., Dias M.K., Kang K.P., Moon S.I., Shin T.S., Ma A., Jung K, & Ahn G. (2022). Hot Water Extract of Sasa borealis (Hack.) Makino & Shibata Abate Hydrogen Peroxide-Induced Oxidative Stress and Apoptosis in Kidney Epithelial Cells. Antioxidants, 11(5), 1013.
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2

Fluorescent Nuclear Morphology Imaging

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Cells were stained with either Hoechst 33342 or mixture of acridine orange and ethidium bromide according to the method described in our previous publication [9 (link)]. Nuclear morphologies were visualized on an EVOS FL Auto 2 Imaging microscope (Thermo-Fisher, Waltham, MA, USA).
Fernando I.P., Dias M.K., Madusanka D.M., Han E.J., Kim M.J., Jeon Y.J., Lee K., Cheong S.H., Han Y.S., Park S.R, & Ahn G. (2020). Human Keratinocyte UVB-Protective Effects of a Low Molecular Weight Fucoidan from Sargassum horneri Purified by Step Gradient Ethanol Precipitation. Antioxidants, 9(4), 340.
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