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Ace excel 2 superc18 column

Manufactured by Phenomenex
Sourced in United Kingdom

The ACE Excel 2 SuperC18 column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of analytes. It features a SuperC18 stationary phase with a particle size of 2.5 micrometers, which provides efficient and reproducible chromatographic separations.

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2 protocols using ace excel 2 superc18 column

1

Quantitative APAP Determination in Plasma

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Acetaminophen (APAP) was extracted from plasma by liquid-liquid extraction using acidified HPLC grade methanol (Fisher Scientific, Loughborough, UK). Briefly, 10μL plasma was enriched with 10ng APAP-d4 (Santa Cruz Biotechnology Inc, Heidelberg, Germany) as internal standard and 0.8mL methanol (w/0.2% acetic acid, Sigma Aldrich, Gillingham, UK) was added, vortexed and incubated for 20 min on ice. After centrifugation (3000g, 10 min, 10 oC), the supernatant was dried under nitrogen at 40 0C, reconstituted in mobile phase (0.2 mL water:methanol 65:35 v/v) and re-centrifuged. Chromatographic separation used an Aria CTC autosampler and Allegros pump on an ACE Excel 2 SuperC18 column (150 × 3 mm; 2 μm) protected by a Kinetex KrudKatcher® (Phenomenex, UK) at 20 oC and detected on a TSQ Quantum Discovery triple quadrupole mass spectrometer (MS; Thermo Fisher Scientific, UK). The mobile phase consisted of 0.1% formic acid (Sigma Aldrich) in aqueous (A) and 0.1% formic acid in methanol (B) at a flow rate of 0.3 mL/min. Gradient elution was achieved with a total run time of 9 minutes from 35% to 5% B. APAP eluted at 3.95 min. The MS was operated in positive ion electrospray mode (300 oC, 3 kV). Transitions monitored were m/z 152→110 and m/z 156.1→114.1 for APAP and APAP-d4, respectively.
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2

Quantitative Acetaminophen Measurement in Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols
Acetaminophen (APAP) was extracted from plasma by liquid-liquid extraction with acidified HPLC-grade methanol (Fisher Scientific). Briefly, 10 μL plasma was enriched with 10 ng deuterium-labelled acetaminophen (APAP-d4; Santa Cruz Biotechnology Inc) as internal standard, 0.8 mL methanol (w/ 0.2% acetic acid, Sigma Aldrich) was added, and the samples were then vortexed and incubated for 20 min on ice. After centrifugation (3000 g, 10 min, 10°C), the supernatant was reduced to dryness under nitrogen at 40°C, reconstituted in mobile phase (200 μL water/methanol (65:35, v/v), and centrifuged for a second time.
Chromatographic separation was achieved using an Aria CTC autosampler and Allegros pump on an ACE Excel 2 SuperC18 column (150 x 3 mm; 2 μm) protected by a Kinetex KrudKatcher (Phenomenex) at 20°C, and acetaminophen was detected on a TSQ Quantum Discovery triple quadrupole mass spectrometer (Thermo Fisher Scientific). The mobile phase consisted of 0.1% formic acid (Sigma Aldrich) in water and 0.1% formic acid in methanol (B) at a flow rate of 0.3 mL/min. Gradient elution was achieved with a total run time of 9 min from 35% to 5% B. APAP eluted at 3.95 mins. The mass spectrometer was operated in positive ion electrospray mode (300°C, 3 kV). Transitions monitored were m/z 152 ➔ 110 and m/z 156.1 ➔ 114.1 for APAP and APAP-d4, respectively.
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