Primary mouse cd68 antibody

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Primary mouse CD68 antibody is a laboratory reagent used for the detection and study of the CD68 protein expressed on the surface of macrophages and monocytes. It can be utilized in various immunological techniques, such as flow cytometry and immunohistochemistry, to identify and characterize these cell types.

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Lab products found in correlation

Alexa fluor 488 coupled goat anti rabbit igg antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 coupled goat anti mouse igg antibody, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Primary mouse antibody (2 citations) CD68 antibody (2 citations)

1 protocol using primary mouse cd68 antibody

Immunofluorescent Detection of Monocytes and Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the detection of monocytes/macrophages, the sections were incubated with the primary mouse CD68 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA). After washing, Alexa Fluor 594‐coupled goat anti‐mouse immunoglobulin G (IgG) antibody (Invitrogen, Carlsbad, CA, USA) and 4′,6‐diamidino‐2‐phenylindole were applied.
To characterize macrophages, the sections were double‐stained with the primary mouse CD68 antibody and rabbit CD206 antibody (Santa Cruz). After washing, Alexa Fluor 594‐coupled goat anti‐mouse IgG antibody, Alexa Fluor 488‐coupled goat anti‐rabbit IgG antibody (Invitrogen) and 4′,6‐diamidino‐2‐phenylindole were applied. Slides were analyzed under a fluorescence microscope.

Omi M., Hata M., Nakamura N., Miyabe M., Kobayashi Y., Kamiya H., Nakamura J., Ozawa S., Tanaka Y., Takebe J., Matsubara T, & Naruse K. (2015). Transplantation of dental pulp stem cells suppressed inflammation in sciatic nerves by promoting macrophage polarization towards anti‐inflammation phenotypes and ameliorated diabetic polyneuropathy. Journal of Diabetes Investigation, 7(4), 485-496.

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