Mice were intraperitoneally injected with D-fluorescein and anesthetized with 1.5% pentobarbital sodium. Optical imaging was detected by Lago X system (Spectral Instruments Imaging).
Lago x system
Manufactured by Spectral Instruments Imaging
Sourced in United States
The Lago X system is a high-performance lab equipment designed for scientific imaging and analysis. It provides advanced capabilities for capturing, processing, and analyzing visual data. The core function of the Lago X system is to enable precise and accurate imaging of samples and specimens, facilitating research and scientific discoveries.
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Lab products found in correlation
6 protocols using lago x system
1
Fluorescein-Guided Optical Imaging in Mice
2
Establishing Subcutaneous and Metastatic Tumor Models in Mice
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Five‐week‐old male BALB/c nude mice were obtained from Vital River Laboratories (Beijing, China) and housed in a specific pathogen‐free environment. All animal studies were approved by the Institutional Animal Use and Care Committee of Tongji Medical College (S1892).
Subcutaneous tumor models were established by subcutaneous injection of a total of 2 × 106 tumor cells in nude mice (n = 5). Subcutaneous tumors were measured every 4 days. Mice were euthanized by CO2 and cervical dislocation on day 44 or when the tumor size exceeded 1.5 cm in diameter, and the tumors were dissected, photographed, and weighed.
Metastatic tumor models were established through tail vein injection of 4 × 106 tumor cells in nude mice (n = 3) to evaluate the metastatic ability of tumor cells. After 8 weeks, nude mice were used for live small animal fluorescent imaging assays by the LagoX system (Spectral instruments imaging, Tucson, AZ, USA).
Subcutaneous tumor models were established by subcutaneous injection of a total of 2 × 106 tumor cells in nude mice (n = 5). Subcutaneous tumors were measured every 4 days. Mice were euthanized by CO2 and cervical dislocation on day 44 or when the tumor size exceeded 1.5 cm in diameter, and the tumors were dissected, photographed, and weighed.
Metastatic tumor models were established through tail vein injection of 4 × 106 tumor cells in nude mice (n = 3) to evaluate the metastatic ability of tumor cells. After 8 weeks, nude mice were used for live small animal fluorescent imaging assays by the LagoX system (Spectral instruments imaging, Tucson, AZ, USA).
3
In Vivo Tumor Targeting of Cy5.5-Labeled Polymer
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The conjugate was labeled with Cy5.5 as follows. In brief, the hydrazide derivative of Cy5.5 was reacted with carboxylic groups of CMD in the presence of EDC overnight in the dark. The reaction mixtures were then dialyzed (molecular weight cut off = 3 kDa) against deionized water to remove non-reacted Cy5.5 and EDC. The purified conjugate was stored at − 20 °C, prior to use. To observe the in vivo tumor-homing ability of the conjugate, a TC-1 flank tumor model was prepared by the subcutaneous inoculation of a cell suspension (1 × 106 cells for each mouse) in RPMI medium (100 μL) into athymic nude mice (6-week-old, female). When the tumor volumes reached 150–200 mm3, 200 μL of physiological saline containing Cy5.5-labeled polymeric conjugate was injected into the tail vein of each mouse. The conjugate was visualized at predetermined time points by scanning the mice using a Lago X system (Spectral Instruments Imaging, Tucson, AZ, USA) with a 670 nm LED. The tumors and major organs (liver, heart, lung, spleen, and kidney) were excised from the mice at 48 h post-injection to quantitatively assess the ex vivo tissue distribution of the conjugate by measuring the average fluorescence intensity over the region of interest.
4
Establishment of Mouse Xenograft Models
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BALB/c nude mice (male, 5‐week‐old) were purchased from Hua Fukang (Beijing, China) and maintained under a specific pathogen‐free barrier environment. The animal study was approved by the Animal Care and Use Committee of Union Hospital (S3694).
The subcutaneous tumor model was created by subcutaneously injecting 2 × 106 CAKI‐1 cells into nude mice (n = 5). At 44 days post‐injection or upon reaching a subcutaneous tumor diameter exceeding 1.5 cm, humane euthanasia was performed using CO2 followed by cervical dislocation. Then the subcutaneous tumors were photographed and weighed.
The metastatic tumor model was established by injecting 4 × 106 A498 cells into the tail veins of nude mice (n = 5). After 8 weeks, the LagoX system (Spectral Instruments Imaging, Tucson, AZ, USA) was applied to visualize the nude mice.
The subcutaneous tumor model was created by subcutaneously injecting 2 × 106 CAKI‐1 cells into nude mice (n = 5). At 44 days post‐injection or upon reaching a subcutaneous tumor diameter exceeding 1.5 cm, humane euthanasia was performed using CO2 followed by cervical dislocation. Then the subcutaneous tumors were photographed and weighed.
The metastatic tumor model was established by injecting 4 × 106 A498 cells into the tail veins of nude mice (n = 5). After 8 weeks, the LagoX system (Spectral Instruments Imaging, Tucson, AZ, USA) was applied to visualize the nude mice.
5
Metastatic Tumor Model in Nude Mice
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Male BALB/c nude mice, aged 6 weeks, were acquired from Vital River Company located in Beijing, China. These mice were kept in an environment that was free from any particular pathogens. All animal experiments were conducted following the guidelines and regulations authorized by the Institutional Animal Ethics Committee of Tongji Medical College (approval no. S1892).
To create a metastatic tumor model, 5×106 A498 cells were intravenously administrated into the vein of nude mice tail (n = 6) to assess the metastatic potential of the tumor cells. After a period of 6 weeks, in vivo fluorescence imaging of the nude mice was conducted using the LagoX system (Spectral Instruments Imaging, Tucson, AZ, USA). After undergoing imaging procedures, the mice were euthanized in a humane manner, and their lungs and livers were then obtained for the purpose of conducting H&E staining.
To create a metastatic tumor model, 5×106 A498 cells were intravenously administrated into the vein of nude mice tail (n = 6) to assess the metastatic potential of the tumor cells. After a period of 6 weeks, in vivo fluorescence imaging of the nude mice was conducted using the LagoX system (Spectral Instruments Imaging, Tucson, AZ, USA). After undergoing imaging procedures, the mice were euthanized in a humane manner, and their lungs and livers were then obtained for the purpose of conducting H&E staining.
6
Quantifying Residual Dye in Mouse Eyes
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