Plant hormones of free SA and JA productions were extracted according to a previously described method [63 (link), 64 (link)]. SA and JA were extracted and quantified according to the method of Liu et al. with appropriate modifications [65 (link)]. Briefly, twig samples (0.5 g for each sample) were immediately frozen in liquid nitrogen and ground with pestle and mortar. The ground samples were extracted with 500 μL modified Bieleski solvent (methanol/H2O, 80/20, v/v) at 4 °C for 12 h. The solutions of SA and JA were prepared as internal standards at a concentration of 1 μg/mL in 100% methanol. All nano-LC experiments were performed on a Shimadzu Prominence nano-flow liquid chromatography system (Kyoto, Japan) with two LC-20 AD nano pumps, two vacuum degassers, a LC-20AB HPLC pump, a SIL-20 AC HT autosampler, and a FCV nano valve. The analytical column of poly (MAA-co-EDMA) monolithic column (100 μm i.d., 360 μm o.d., 30-cm long, purchased from Weltech Co., Ltd., Wuhan, China) was connected to the nano-LC system and conditioned with the mobile phase (ACN/H2O, 50/50, v/v) at a flow rate of 600 μL/min for 30 min.
Lc 20ab hplc pump
Manufactured by Shimadzu
Sourced in Japan
The LC-20AB HPLC pump is a high-performance liquid chromatography (HPLC) pump manufactured by Shimadzu. It is designed to deliver a consistent and accurate flow of mobile phase to the HPLC system.
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4 protocols using lc 20ab hplc pump
1
Quantifying Plant Hormone Levels
2
Extraction and Quantification of Plant Hormones
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Plant hormones of free SA and JA productions were extracted according to a previously described method [65, 66] . SA and JA were extracted and quanti ed according to the method of Liu et al. with appropriate modi cations [67] . Brie y, twig samples (0.5 g for each sample) were immediately frozen in liquid nitrogen and ground with pestle and mortar. The ground samples were extracted with 500 μL modi ed Bieleski solvent (methanol/H 2 O, 80/20, v/v) at 4 °C for 12 h. The solutions of SA and JA were prepared as internal standards at a concentration of 1 μg/mL in 100% methanol. All nano-LC experiments were performed on a Shimadzu Prominence nano-ow liquid chromatography system (Kyoto, Japan) with two LC-20AD nano pumps, two vacuum degassers, a LC-20AB HPLC pump, a SIL-20AC HT autosampler, and a FCV nano valve. The analytical column of poly (MAA-co-EDMA) monolithic column (100 μm i.d., 360 μm o.d., 30-cm long, purchased from Weltech Co., Ltd., Wuhan, China) was connected to the nano-LC system and conditioned with the mobile phase (ACN/H 2 O, 50/50, v/v) at a ow rate of 600 μL/min for 30 min.
3
Extraction and Quantification of Plant Hormones
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plant hormones of free SA and JA productions were extracted according to a previously described method [65, 66] . SA and JA were extracted and quanti ed according to the method of Liu et al. with appropriate modi cations [67] . Brie y, twig samples (0.5 g for each sample) were immediately frozen in liquid nitrogen and ground with pestle and mortar. The ground samples were extracted with 500 μL modi ed Bieleski solvent (methanol/H 2 O, 80/20, v/v) at 4 °C for 12 h. The solutions of SA and JA were prepared as internal standards at a concentration of 1 μg/mL in 100% methanol. All nano-LC experiments were performed on a Shimadzu Prominence nano-ow liquid chromatography system (Kyoto, Japan) with two LC-20AD nano pumps, two vacuum degassers, a LC-20AB HPLC pump, a SIL-20AC HT autosampler, and a FCV nano valve. The analytical column of poly (MAA-co-EDMA) monolithic column (100 μm i.d., 360 μm o.d., 30-cm long, purchased from Weltech Co., Ltd., Wuhan, China) was connected to the nano-LC system and conditioned with the mobile phase (ACN/H 2 O, 50/50, v/v) at a ow rate of 600 μL/min for 30 min.
4
Quantification of Plant Hormones SA and JA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plant hormones of free SA and JA productions were extracted according to a previously described method [62, 63] . SA and JA were extracted and quanti ed according to the method of Liu et al. with appropriate modi cations [64] . Brie y, twig samples (0.5 g for each sample) were immediately frozen in liquid nitrogen and ground with pestle and mortar. The ground samples were extracted with 500 µL modi ed Bieleski solvent (methanol/H 2 O, 80/20, v/v) at 4 °C for 12 h. The solutions of SA and JA were prepared as internal standards at a concentration of 1 µg/mL in 100% methanol. All nano-LC experiments were performed on a Shimadzu Prominence nano-ow liquid chromatography system (Kyoto, Japan) with two LC-20AD nano pumps, two vacuum degassers, a LC-20AB HPLC pump, a SIL-20AC HT autosampler, and a FCV nano valve. The analytical column of poly (MAA-co-EDMA) monolithic column (100 µm i.d., 360 µm o.d., 30-cm long, purchased from Weltech Co., Ltd., Wuhan, China) was connected to the nano-LC system and conditioned with the mobile phase (ACN/H 2 O, 50/50, v/v) at a ow rate of 600 µL/min for 30 min.
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