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Alexa 647 anti cd49f antibody

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Alexa 647-anti CD49f antibody is a fluorescently-labeled monoclonal antibody that specifically binds to the CD49f antigen. CD49f is a subunit of the alpha 6 integrin and is expressed on various cell types.

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Lab products found in correlation

Lsrfortessa, by BD (4 mentions) Lsr 2, by BD (2 mentions) Aria 1, by BD (2 mentions) Pe or pe cy7 anti sca 1 antibody, by Thermo Fisher Scientific (2 mentions) Anti cd11b pacific blue, by Thermo Fisher Scientific (2 mentions) Aria 2, by BD (2 mentions) Pe anti sca 1 antibody, by Thermo Fisher Scientific (2 mentions) Pe cy7 anti gr 1 antibodies, by BioLegend (2 mentions)

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Alexa 647 (11 citations)

4 protocols using alexa 647 anti cd49f antibody

1

Prostate Cell Lineage Separation

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For the separation of prostate cell lineages, dissociated murine prostate cells were stained with FITC- or Pacific blue-anti CD31, CD45 and Ter119 antibodies (eBioscience, San Diego, CA)], PE- or PE-Cy7-anti Sca-1 antibody (eBioscience, San Diego, CA) and Alexa 647-anti CD49f antibody (Biolegend, San Diego, CA). FACS analyses and sorting were performed by using the BD LSR II or BD LSR Fortessa and Aria I, respectively (BD Biosciences, San Jose, CA).
Kwon O.J., Valdez J., Zhang L., Zhang B., Wei X., Su Q., Ittmann M.M., Creighton C.J, & Xin L. (2014). Increased Notch signaling inhibits anoikis and stimulates proliferation of prostate luminal epithelial cells. Nature communications, 5, 4416.
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2

Isolation of Murine Prostate Stromal Cells

Cited 1 time
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For separating prostate stromal cells, dissociated murine prostate cells were prepared as described previously (15 (link)) and stained with Pacific blue-anti CD31, CD45 and Ter119 antibodies (eBioscience, San Diego, CA), PE-anti Sca-1 antibody (eBioscience, San Diego, CA), Alexa 647-anti CD49f antibody (Biolegend, San Diego, CA). Prostate stromal cells are CD31−CD45−Ter119−Sca-1+CD49f− (48 (link)). For detecting MDSC cells, dissociated murine prostate cells were stained with Pacific blue-anti CD11b (eBioscience, San Diego, CA), and PE-Cy7-anti Gr-1 antibodies (Biolegend, San Diego, CA). FACS analyses and sorting were performed by using the BD LSR Fortessa and Aria II, respectively (BD Biosciences, San Jose, CA).
Su Q., Zhang B., Zhang L., Dang T., Rowley D., Ittmann M, & Xin L. (2016). Jagged1 upregulation in prostate epithelial cells promotes formation of reactive stroma in the Pten null mouse model for prostate cancer. Oncogene, 36(5), 618-627.
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3

Isolation of Murine Prostate Stromal Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For separating prostate stromal cells, dissociated murine prostate cells were prepared as described previously (15 (link)) and stained with Pacific blue-anti CD31, CD45 and Ter119 antibodies (eBioscience, San Diego, CA), PE-anti Sca-1 antibody (eBioscience, San Diego, CA), Alexa 647-anti CD49f antibody (Biolegend, San Diego, CA). Prostate stromal cells are CD31−CD45−Ter119−Sca-1+CD49f− (48 (link)). For detecting MDSC cells, dissociated murine prostate cells were stained with Pacific blue-anti CD11b (eBioscience, San Diego, CA), and PE-Cy7-anti Gr-1 antibodies (Biolegend, San Diego, CA). FACS analyses and sorting were performed by using the BD LSR Fortessa and Aria II, respectively (BD Biosciences, San Jose, CA).
Su Q., Zhang B., Zhang L., Dang T., Rowley D., Ittmann M, & Xin L. (2016). Jagged1 upregulation in prostate epithelial cells promotes formation of reactive stroma in the Pten null mouse model for prostate cancer. Oncogene, 36(5), 618-627.
+ Open protocol
+ Expand
4

Prostate Cell Lineage Separation

Check if the same lab product or an alternative is used in the 5 most similar protocols
For the separation of prostate cell lineages, dissociated murine prostate cells were stained with FITC- or Pacific blue-anti CD31, CD45 and Ter119 antibodies (eBioscience, San Diego, CA)], PE- or PE-Cy7-anti Sca-1 antibody (eBioscience, San Diego, CA) and Alexa 647-anti CD49f antibody (Biolegend, San Diego, CA). FACS analyses and sorting were performed by using the BD LSR II or BD LSR Fortessa and Aria I, respectively (BD Biosciences, San Jose, CA).
Kwon O.J., Valdez J., Zhang L., Zhang B., Wei X., Su Q., Ittmann M.M., Creighton C.J, & Xin L. (2014). Increased Notch signaling inhibits anoikis and stimulates proliferation of prostate luminal epithelial cells. Nature communications, 5, 4416.
+ Open protocol
+ Expand

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