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Clone 1c10

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Clone 1C10 is a monoclonal antibody that recognizes the human CD8 antigen. CD8 is a glycoprotein expressed on the surface of cytotoxic T cells and a subset of natural killer cells. The clone 1C10 antibody can be used for the identification and enumeration of CD8+ T cells by flow cytometry.

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Lab products found in correlation

Anti cd40, by BioLegend (2 mentions) Rmil 4, by Thermo Fisher Scientific (2 mentions) Rmil 5, by Thermo Fisher Scientific (1 mentions) Anti cd40 clone 1c10, by BioLegend (1 mentions) Rmil 4, by Merck Group (1 mentions) Lps from e coli o55 b5, by Merck Group (1 mentions) Easysep mouse b cell isolation kit, by STEMCELL (1 mentions)

Spelling variants of this product

Anti-CD40 (clone 1C10, (3 citations)

2 protocols using clone 1c10

1

Optimizing B Cell Class Switching

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B cells were isolated with the EasySep Mouse B cell isolation kit (Stem Cell Technology, Canada) from total splenocytes. To induce CSR from IgM to IgG1 or IgE, B cells (5×105−1×106 cells/ mL) were activated with 1 μg/mL anti-CD40 clone 1C10, Biolegend) and rmIL-4 (10 ng/mL, Peprotech). For IgG3 and IgG2b switching, B cells were activated with 25 ug/mL of LPS from E. coli O55:B5 (Sigma, St. Louis, MO) and 10 ng/mL rmIL-4 at 37°C 5% CO 2. For IgA and IgG1 switching, B cells were activated with anti-CD40 (1 μg/mL, clone 1C10, Biolegend), rmIL-4 (10 ng/mL, Peprotech), rmIL-5 (10ng/mL, Peprotech), and rhTGFβ (1 ng/mL).
Shukla V., Halabelian L., Balagere S., Samaniego-Castruita D., Feldman D.E., Arrowsmith C.H., Rao A, & Aravind L. (2019). HMCES functions in the alternative end-joining pathway of the DNA DSB repair during class switch recombination in B cells.. Molecular cell, 77(2), 384-394.e4.
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2

Class Switch Recombination in CH12 Cells

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For CSR from IgM to IgA in CH12 cell line, cells were plated at a density of 50X103 per mL of media and stimulated with anti-CD40 (1 μg/mL, clone 1C10, Biolegend), rmIL-4 (10 ng/mL, Peprotech), and rhTGFβ (1 ng/mL) (CIT) for 72 hours or the indicated time-points.
Shukla V., Halabelian L., Balagere S., Samaniego-Castruita D., Feldman D.E., Arrowsmith C.H., Rao A, & Aravind L. (2019). HMCES functions in the alternative end-joining pathway of the DNA DSB repair during class switch recombination in B cells.. Molecular cell, 77(2), 384-394.e4.
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