All chemicals were purchased from Sigma-Aldrich and used as-is. Bovine liver catalase was also purchased from Sigma-Aldrich. Hydrogen peroxide solution (9.79 M) TraceSELECT Ultra was purchased from Fluka. Dimedone was purchased from Acros. NaSH was purchased from Alfa Aesar in its anhydrous form and stored in a glove box (<1 p.p.m. O2 and <1 p.p.m. H2O). Stock solutions of NaSH were prepared immediately before use in a buffer (50 mM phosphate buffer at pH 7.4, 1 mM DTPA) devoid of trace elements and degassed with argon.
Dimedone
Manufactured by Thermo Fisher Scientific
Dimedone is a laboratory reagent used as a colorimetric indicator for the detection and quantification of aldehydes and ketones. It functions by forming a yellow-colored complex when reacting with these carbonyl compounds. Dimedone is commonly used in analytical chemistry applications that require the identification and measurement of carbonyl-containing substances.
Automatically generated - may contain errors
Lab products found in correlation
Bovine liver catalase, by Merck Group (1 mentions)
Acetophenone, by Thermo Fisher Scientific (1 mentions)
Benzaldehyde, by Merck Group (1 mentions)
4 fluorobenzaldehyde, by Merck Group (1 mentions)
4 methylbenzaldehyde, by Merck Group (1 mentions)
2 furaldehyde, by Merck Group (1 mentions)
4 chlorobenzaldehyde, by Merck Group (1 mentions)
2 chlorobenzaldehyde, by Merck Group (1 mentions)
4 bromobenzaldehyde, by Merck Group (1 mentions)
4 methoxybenzaldehyde, by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
Silica gel, by Merck Group (1 mentions)
2 protocols using dimedone
1
Catalase-Mediated Hydrogen Peroxide Assay
2
Benzaldehyde Derivatives Synthesis Protocols
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Benzaldehyde (99%), 4-methoxyBenzaldehyde (98%), 4-chloroBenzaldehyde (98%), 4-fluoroBenzaldehyde (98%), 2-fluoroBenzaldehyde (97%), 4-bromoBenzaldehyde (99%), 4-methylBenzaldehyde (97%), 2-furaldehyde (99%), ammonium acetate (97%), 2-chloroBenzaldehyde (99%) were purchased from Sigma-Aldrich. 1,3-Cyclohexadione (97%), acetophenone (98%), malononitrile (99%), and dimedone (99%) were acquired from Acros.
Ethyl acetate (for analysis EMSURE ACS, ISO, Reag. Ph Eur) and acetone (for analysis EMPARTA ACS) were acquired from Merck. Thin-layer chromatography (TLC) was conducted using aluminum plates (F-254) covered with silica gel. The experiment included the use of silica gel (230–400 mesh, Merck) for column chromatography.
Ethyl acetate (for analysis EMSURE ACS, ISO, Reag. Ph Eur) and acetone (for analysis EMPARTA ACS) were acquired from Merck. Thin-layer chromatography (TLC) was conducted using aluminum plates (F-254) covered with silica gel. The experiment included the use of silica gel (230–400 mesh, Merck) for column chromatography.
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