Trypsin ethylenediaminetetraacetic acid edta

Hormones (I, C, P, E1, E2, and EGF) were purchased from Sigma-Aldrich (St. Louis, MO, USA). L-Arginine (Arg), L-histidine (His), L-leucine (Leu), L-isoleucine (Ile), L-threonine (Thr), L-tryptophan (Trp), L-lysine (Lys), L-methionine (Met), L-phenylalanine (Phe), and L-valine (Val) were purchased from Sigma-Aldrich (USA). Dulbecco’s modified Eagle medium/nutrient mixture F12 (DMEM/F12) was obtained from Gibco (Grand Island, NY, USA). Fetal bovine serum (FBS), penicillin/streptomycin (P/S), and trypsin-ethylenediaminetetraacetic acid (EDTA) solutions were purchased from WELGENE Inc. (Gyeongsan, Daegu, Korea). Phosphate-buffered saline (PBS) was obtained from Lonza (Walkersville, MD, USA).

Silymarin, B[a]P, dimethyl sulfoxide (DMSO), 4,6-diamidino-2-phenylindole dihydrochloride (DAPI), and Triton X-100 were purchased from Sigma-Aldrich Chemical (St. Louis, MO, USA). Minimum essential medium (MEM), fetal bovine serum (FBS), penicillin/streptomycin, trypsin-ethylenediaminetetraacetic acid (EDTA), and sodium pyruvate were purchased from Welgene (Daegu, Korea). Phosphate-buffered saline was purchased from Biosesang (Seongnam, Korea). Fluorescent mounting medium was purchased from Dako (Carpinteria, CA, USA). Antibodies (anti-Nrf2, PXR, GST, CYP1A1, ABCC1, and β-actin), horseradish peroxidase-conjugated anti-rabbit immunoglobulin G (IgG) and siRNA transfection reagent, siRNA transfection medium, control siRNA, and Nrf2-siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Alexa 488-conjugated anti-rabbit secondary antibody was purchased from Cell Signaling Technology (Beverly, MA, USA).