Red sacaca leaves were collected from the medicinal plants sector of Embrapa Amazônia Ocidental, (localization 03°06′23,04″S e 60°01′35,14″W) in Manaus, Amazonas, Brazil. The access to the botanical material was registered on the National System of Management of Genetic Heritage and Associated Traditional Knowledge (SISGEN) under the number AD070A3. The red sacaca essential oil was obtained by hydrodistillation in a modified Clevenger apparatus (LCR Cientifica; Cosmopolis, Brasil) for 4 h, from plants collected between 08:00–09:00 a.m., as reported previously (Azevedo et al., 2016 (link); Silva et al., 2022 (link)).
Compritol® 888 ATO was kindly supplied by Gattefossé (Lyon, France), Super refined Tween® 80 (polysorbate 80) was kindly provided by Croda Inc. (Edison, WI, United States), and soybean lecithin was obtained from Cargill (Krefeld, Germany). Butyl hydroxytoluene (BHT) was acquired from Sulfal (Belo Horizonte, Brazil). The reagents 2,2-diphenyl-1-picrylhydrazyl (DPPH∙), 7-hydroxy-3H-phenoxazin-3-one 10-oxide (resazurin) and 2ʹ,7ʹ-diclorofluorescein diacetate (DCFDA) were acquired from Sigma-Aldrich (St. Louis, MO, United States). All the other reagents were of analytical grade.
Compritol® 888 ATO was kindly supplied by Gattefossé (Lyon, France), Super refined Tween® 80 (polysorbate 80) was kindly provided by Croda Inc. (Edison, WI, United States), and soybean lecithin was obtained from Cargill (Krefeld, Germany). Butyl hydroxytoluene (BHT) was acquired from Sulfal (Belo Horizonte, Brazil). The reagents 2,2-diphenyl-1-picrylhydrazyl (DPPH∙), 7-hydroxy-3H-phenoxazin-3-one 10-oxide (resazurin) and 2ʹ,7ʹ-diclorofluorescein diacetate (DCFDA) were acquired from Sigma-Aldrich (St. Louis, MO, United States). All the other reagents were of analytical grade.