The AgNO3 (≥99.0%), NaBH4 (≥99%), cresyl violet perchlorate (>96.5%), ammonium acetate (≥99%), and sodium acetate (≥99%) were purchased from Sigma Aldrich and used without further purification. The ethanol was purchased from ECHO Chemical, and the HPLC purified d[T2C3AC3AC4G2C3], d[CGC6T2G2CGT] and d[AG3(T2AG3)3] DNA were obtained from MDBio. The TEMED (N,N,N′,N′-tetramethylethylenediamine), ammonium persulfate, loading buffer (0.03% Xylene cyanol FF, 0.03% Bromophenol blue, 10 mM Tris–HCl (pH8.0), 60% glycerol, 60 mM EDTA) and the 10× TBE pH = 8.0 buffer were obtained from Biomate. The 19 : 1 (w/w) acrylamide/bisacrylamide was obtained from Bio Basic. Inc. The SYBR gold was obtained from Fisher scientific. Deionized water (resistivity >18.2 MΩ cm) was obtained from Barnstead™ EasyPure™ II water purification system (Fisher Scientific).
Cresyl violet perchlorate
Manufactured by Merck Group
Sourced in United States
Cresyl violet perchlorate is a synthetic dye used in various laboratory applications. It is a crystalline powder that is soluble in water and certain organic solvents. The primary function of cresyl violet perchlorate is as a staining agent for microscopy and histological techniques.
Automatically generated - may contain errors
Lab products found in correlation
Nabh4, by Merck Group (1 mentions)
Sodium acetate, by Merck Group (1 mentions)
Sybr gold, by Thermo Fisher Scientific (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Agno3, by Merck Group (1 mentions)
Stemi 508, by Zeiss (1 mentions)
Axiocam 105 color, by Zeiss (1 mentions)
Zen digital imaging, by Zeiss (1 mentions)
2 protocols using cresyl violet perchlorate
1
Polyacrylamide Gel Electrophoresis of DNA
2
Histological Verification of VTA and PPN
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All animals were killed two hours after the end of Es-VTA (pentobarbital, 0.9% saline, phosphate-buffered 4% formaldehyde).
To determine the placement of the electrode in the VTA and cannula in the PPN, Nissl staining in select sections was performed from 4.52 to 5.60 mm and from 7.30 to 8.30 mm sections (the thickness of each section was 20 µm). The sections were mounted on gelatin-coated slides, stained with Cresyl violet perchlorate (Sigma-Aldrich, St. Louis, MO, USA), and dehydrated, and coverslips were placed on top using DPX for histology (Sigma-Aldrich, St. Louis, MO, USA). Micro photos of the sections were taken with simultaneous application of templates derived from the brain atlas of rats [28 ] using a magnifier (Stemi 508; Zeiss, Oberkochen, Germany) and camera (Axiocam 105 color; Zeiss) with integrated software (Zen Digital Imaging; Zeiss, Oberkochen, Germany).
To determine the placement of the electrode in the VTA and cannula in the PPN, Nissl staining in select sections was performed from 4.52 to 5.60 mm and from 7.30 to 8.30 mm sections (the thickness of each section was 20 µm). The sections were mounted on gelatin-coated slides, stained with Cresyl violet perchlorate (Sigma-Aldrich, St. Louis, MO, USA), and dehydrated, and coverslips were placed on top using DPX for histology (Sigma-Aldrich, St. Louis, MO, USA). Micro photos of the sections were taken with simultaneous application of templates derived from the brain atlas of rats [28 ] using a magnifier (Stemi 508; Zeiss, Oberkochen, Germany) and camera (Axiocam 105 color; Zeiss) with integrated software (Zen Digital Imaging; Zeiss, Oberkochen, Germany).
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